Overproduction of trehalose

Simple item page
dc.contributor.authorPadilla, L
dc.contributor.authorKrämer, R
dc.contributor.authorStephanopoulos, G
dc.contributor.authorAgosin, E
dc.date.accessioned2025-01-21T01:08:32Z
dc.date.available2025-01-21T01:08:32Z
dc.date.issued2004
dc.description.abstractTrehalose is a disaccharide with potential applications in the biotechnology and food industries. We propose a method for industrial production of trehalose, based on improved strains of Corynebacterium glutamicum. This paper describes the heterologous expression of Escherichia coli trehalose-synthesizing enzymes trehalose-6-phosphate synthase (OtsA) and trehalose-6-phosphate phosphatase (OtsB) in C. glutamicum, as well as its impact on the trehalose biosynthetic rate and metabolic-flux distributions, during growth in a defined culture medium. The new recombinant strain showed a five- to sixfold increase in the activity of OtsAB pathway enzymes, compared to a control strain, as well as an almost fourfold increase in the trehalose excretion rate during the exponential growth phase and a twofold increase in the final titer of trehalose. The heterologous expression described resulted in a reduced specific glucose uptake rate and Krebs cycle flux, as well as reduced pentose pathway flux, a consequence of downregulated glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. The results proved the suitability of using the heterologous expression of Ots proteins in C. glutamicum to increase the trehalose biosynthetic rate and yield and suggest critical points for further improvement of trehalose overproduction in C. glutamicum.
dc.fuente.origenWOS
dc.identifier.doi10.1128/AEM.70.1.370-376.2004
dc.identifier.eissn1098-5336
dc.identifier.issn0099-2240
dc.identifier.urihttps://doi.org/10.1128/AEM.70.1.370-376.2004
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96479
dc.identifier.wosidWOS:000188115300047
dc.issue.numero1
dc.language.isoen
dc.pagina.final376
dc.pagina.inicio370
dc.revistaApplied and environmental microbiology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.ods12 Responsible Consumption and Production
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa12 Producción y consumo responsable
dc.titleOverproduction of trehalose
dc.typeartículo
dc.volumen70
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas