Biliary aminopeptidase-N and the cholesterol crystallization defect in cholelithiasis

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dc.catalogadoryvc
dc.contributor.authorNúñez, L.
dc.contributor.authorAmigo Boker, Ludwig Peter
dc.contributor.authorMingrone, G.
dc.contributor.authorRigotti Rivera, Attilio Gianpietro
dc.contributor.authorPuglielli, L.
dc.contributor.authorRaddatz Echavarría, Alejandro
dc.contributor.authorPimentel González, Eduardo Fernando
dc.contributor.authorGreco, A.
dc.contributor.authorGonzález, S.
dc.contributor.authorGarrido Negri, Jorge
dc.contributor.authorMiquel Poblete, Juan Francisco
dc.contributor.authorNervi Oddone, Flavio
dc.contributor.otherPontificia Universidad Católica de Chile. Departamento de Gastroenterología y Centro para la Prevención y tratamiento del Cáncer Digestivo
dc.contributor.otherInstitute of Clinical Medicine, Universita Cattolica del Sacro Cuore, Rome, Italy
dc.date.accessioned2024-08-09T15:34:36Z
dc.date.available2024-08-09T15:34:36Z
dc.date.issued1995
dc.description.abstractSeveral biliary proteins have cholesterol crystallisation promoting activity. One of these glycoproteins is aminopeptidase-N, a canalicular ectoenzyme. This study attempted to localise aminopeptidase-N along the biliary tree, to assess its concentration in a series of 98 patients subjected to abdominal surgery, 40 of them without gap stones, and to correlate its concentration with cholesterol crystal formation time of gall bladder bile. Aminopeptidase-N was isolated from purified native biliary vesicles. A specific polyclonal rabbit anti-aminopeptidase-N antibody was prepared for quantitative immunoblotting and for immunolocalisation. Tissue was obtained from liver biopsy specimens and from gall bladders removed at surgery because of gall stone disease. Aminopeptidase-N was immunolocalised to the apical membranes of hepatocytes and to the apical pole of ductular and gall bladder mucosal cells. The nucleation time of gall bladder bile was mean (SD) 4 (3) days in the gall stone group, compared with 21 (18) days in the control group (p < 0.001). Total absolute biliary protein and aminopeptidase-N concentrations were similar in both the control and gall stone patients. There was a reciprocal significant correlation, however, between the nucleation time and the relative aminopeptidase-N concentration (r = -0.35, p < 0.01) only in the gall stone group of patients, This study shows that this apical transmembrane ectoenzyme with cholesterol crystallisation promoting activity is present along the biliary tree and the hepatocyte. These findings support the concept that high concentrations or qualitative changes of biliary aminopeptidase-N contribute to cholesterol gall stone formation.
dc.fechaingreso.objetodigital2024-08-09
dc.format.extent5 páginas
dc.fuente.origenWoS
dc.identifier.doi10.1136/gut.37.3.422
dc.identifier.issn0017-5749
dc.identifier.pubmedidMedline_ID: 7590442
dc.identifier.urihttps://doi.org/10.1136/gut.37.3.422
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/87391
dc.identifier.wosidWOS:A1995RT37100023
dc.information.autorucEscuela de Medicina; Amigo Boker, Ludwig Peter; S/I; 62028
dc.information.autorucEscuela de Medicina; Rigotti Rivera, Attilio Gianpietro; 0000-0002-0495-3525; 68489
dc.information.autorucEscuela de Medicina; Raddatz Echavarría, Alejandro; S/I; 99184
dc.information.autorucEscuela de Medicina; Pimentel González, Eduardo Fernando; S/I; 205651
dc.information.autorucEscuela de Medicina; Garrido Negri, Jorge; S/I; 97915
dc.information.autorucEscuela de Medicina; Miquel Poblete, Juan Francisco; 0000-0002-0526-4377; 72216
dc.information.autorucEscuela de Medicina; Nervi Oddone, Flavio; 0000-0002-6642-7985; 99156
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final426
dc.pagina.inicio422
dc.publisherBritish Medical Journal Publishing Group
dc.revistaGut
dc.rightsacceso restringido
dc.subjectBiliary Cholesterol
dc.subjectCrystallization
dc.subjectAminopeptidase-N
dc.subjectGall Stones
dc.subjectNucleation Promoting Activity
dc.subjectHuman Gallbladder Bile
dc.subjectGallstone Disease
dc.subjectPhospholipase-C
dc.subjectModel Bile
dc.subjectImmunoglobulins
dc.subjectProteins
dc.subjectPathogenesis
dc.subjectGlycoprotein
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleBiliary aminopeptidase-N and the cholesterol crystallization defect in cholelithiasis
dc.typeartículo
dc.volumen37
sipa.codpersvinculados62028
sipa.codpersvinculados68489
sipa.codpersvinculados99184
sipa.codpersvinculados205651
sipa.codpersvinculados97915
sipa.codpersvinculados72216
sipa.codpersvinculados99156
sipa.trazabilidadWoS;05-06-2021
sipa.trazabilidadHistorial Académico;09-07-2021
sipa.trazabilidadORCID;2024-08-05
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