Autophagy Activation in Zebrafish Heart Regeneration

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dc.contributor.authorChavez, Myra N.
dc.contributor.authorMorales, Rodrigo A.
dc.contributor.authorLopez-Crisosto, Camila
dc.contributor.authorCarlos Roa, Juan
dc.contributor.authorAllende, Miguel L.
dc.contributor.authorLavandero, Sergio
dc.date.accessioned2025-01-23T19:54:58Z
dc.date.available2025-01-23T19:54:58Z
dc.date.issued2020
dc.description.abstractAutophagy is an evolutionarily conserved process that plays a key role in the maintenance of overall cellular health. While it has been suggested that autophagy may elicit cardioprotective and pro-survival modulating functions, excessive activation of autophagy can also be detrimental. In this regard, the zebrafish is considered a hallmark model for vertebrate regeneration, since contrary to adult mammals, it is able to faithfully regenerate cardiac tissue. Interestingly, the role that autophagy may play in zebrafish heart regeneration has not been studied yet. In the present work, we hypothesize that, in the context of a well-established injury model of ventricular apex resection, autophagy plays a critical role during cardiac regeneration and its regulation can directly affect the zebrafish regenerative potential. We studied the autophagy events occurring upon injury using electron microscopy, in vivo tracking of autophagy markers, and protein analysis. Additionally, using pharmacological tools, we investigated how rapamycin, an inducer of autophagy, affects regeneration relevant processes. Our results show that a tightly regulated autophagic response is triggered upon injury and during the early stages of the regeneration process. Furthermore, treatment with rapamycin caused an impairment in the cardiac regeneration outcome. These findings are reminiscent of the pathophysiological description of an injured human heart and hence put forward the zebrafish as a model to study the poorly understood double-sword effect that autophagy has in cardiac homeostasis.
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41598-020-59106-z
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/s41598-020-59106-z
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100698
dc.identifier.wosidWOS:000560688100007
dc.issue.numero1
dc.language.isoen
dc.revistaScientific reports
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAutophagy Activation in Zebrafish Heart Regeneration
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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