Stable transformation of <i>Pinus radiata</i> embryogenic tissue by <i>Agrobacterium tumefaciens</i>

Simple item page
dc.contributor.authorCerda, F
dc.contributor.authorAquea, F
dc.contributor.authorGebauer, M
dc.contributor.authorMedina, C
dc.contributor.authorArce-Johnson, P
dc.date.accessioned2025-01-21T01:10:37Z
dc.date.available2025-01-21T01:10:37Z
dc.date.issued2002
dc.description.abstractEmbryogenic cultures from immature zygotic embryos of Pinus radiata seeds were established on semisolid proliferation medium with 2,4-D and BAP. Growing embryogenic masses containing embryonal cells and suspensor cells were subcultured on this media every 2 weeks. After 10 weeks, embryogenic masses (1.5 cm diameter) were transferred to a maturation medium containing ABA. Fully developed somatic embryos were obtained in this medium after 12 weeks. Embryogenic masses were genetically transformed using Agrobacterium tumefaciens. The pBI121 vector containing beta-glucuronidase (uidA) and the neomycin phosphotransferase (nptll) genes was introduced into this tissue. After co-cultivation with Agrobacterium, the embryogenic tissues were transferred to a selection media containing geneticin and carbenicillin. After 1 month of selection, histochemical assays showed extensive GUS positive activity zones in the transformed embryogenic tissues. Under light microscope, blue crystals were seen inside the embryogenic and suspensor cells, and also completely blue somatic embryos were obtained. The uidA gene was also detected by PCR analysis in genomic DNA isolated from transformed embryogenic tissues. These results indicate stable transformation of P. radiata somatic embryogenic tissues using Agrobacterium-mediated transformation.
dc.fuente.origenWOS
dc.identifier.eissn1573-5044
dc.identifier.issn0167-6857
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96702
dc.identifier.wosidWOS:000177102500004
dc.issue.numero3
dc.language.isoen
dc.pagina.final257
dc.pagina.inicio251
dc.revistaPlant cell tissue and organ culture
dc.rightsacceso restringido
dc.subjectimmature zygotic embryos
dc.subjectbeta-glucuronidase
dc.subjectMonterrey pine
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleStable transformation of <i>Pinus radiata</i> embryogenic tissue by <i>Agrobacterium tumefaciens</i>
dc.typeartículo
dc.volumen70
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas