D-Glucose increases the expression and activity of hCAT-1 and Spl binding to SLC7A1 promoter in human umbilical vein endothelium

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dc.catalogadorpau
dc.contributor.authorGonzález Ortiz, Marcelo Andrés
dc.contributor.authorFarías Jofré, Marcelo Enrique
dc.contributor.authorCasanello Toledo, Paola Cecilia
dc.contributor.authorSobrevía Luarte, Luis Alberto
dc.date.accessioned2023-05-18T19:49:48Z
dc.date.available2023-05-18T19:49:48Z
dc.date.issued2008
dc.description.abstractL-Arginine is mainly transported by the human cationic amino acid transporter 1 (hCAT-1, protein codified by the gene SLC7A1) in human umbilical vein endothelial cells (HUVEC). hCAT-1 mediated L-arginine transport and nitric oxide (NO) synthesis are increased by 25 mM D-glucose in this cell type. Since Sp1 is a transcription factor activated by NO, we studied whether high D-glucose effect on transport was due to altered expression of hCAT-1 and abundance and activity of the transcription factor Sp1 in primary cultures of HUVEC. D-Glucose (25 mM, 24 hours) increased (~3.2 fold) the maximal velocity (Vmax), without altering the apparent Km, of L-arginine transport compared with cells in 5 mM D-glucose. High D-glucose also increased the hCAT-1 mRNA number of copies (~5-fold) and protein abundance (~2-fold), and Sp1 nuclear abundance and binding to SLC7A1 promoter region (−177 to −103 bp from ATG). Thus, the stimulatory effect of D-glucose on L-arginine transport could result from increased expression of hCAT-1 due to increased activity of Sp1 on the promoter of SLC7A1 in HUVEC.
dc.format.extent1 página
dc.fuente.origenORCID
dc.identifier.doi10.1096/fasebj.22.1_supplement.964.4
dc.identifier.issn1530-6860
dc.identifier.urihttps://faseb.onlinelibrary.wiley.com/doi/10.1096/fasebj.22.1_supplement.964.4
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/67124
dc.information.autorucFacultad de Ciencias Biológicas ; González Ortiz, Marcelo Andrés ; 0000-0002-0521-3633 ; 158187
dc.information.autorucEscuela de Medicina ; Farías Jofré, Marcelo Enrique ; 0000-0003-0473-2295 ; 12286
dc.information.autorucEscuela de Medicina ; Casanello Toledo, Paola Cecilia ; 0000-0002-2355-1476 ; 146772
dc.information.autorucEscuela de Medicina ; Sobrevía Luarte, Luis Alberto ; 0000-0001-5802-2243 ; 1002656
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final964,4
dc.pagina.inicio964,4
dc.relation.ispartofThe Experimental Biology (2008 ; San Diego, United States of America)
dc.revistaThe FASEB Journal
dc.rightsacceso restringido
dc.titleD-Glucose increases the expression and activity of hCAT-1 and Spl binding to SLC7A1 promoter in human umbilical vein endothelium
dc.typeartículo
dc.volumen22
sipa.codpersvinculados158187
sipa.codpersvinculados12286
sipa.codpersvinculados146772
sipa.codpersvinculados1002657
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