Over-expression of muscle glycogen synthase in human diabetic nephropathy

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dc.contributor.authorGatica, Rodrigo
dc.contributor.authorBertinat, Romina
dc.contributor.authorSilva, Pamela
dc.contributor.authorKairath, Pamela
dc.contributor.authorSlebe, Felipe
dc.contributor.authorPardo, Fabian
dc.contributor.authorRamirez, Maria J.
dc.contributor.authorSlebe, Juan C.
dc.contributor.authorCampistol, Jose M.
dc.contributor.authorNualart, Francisco
dc.contributor.authorCaelles, Carme
dc.contributor.authorYanez, Alejandro J.
dc.date.accessioned2025-01-23T21:38:08Z
dc.date.available2025-01-23T21:38:08Z
dc.date.issued2015
dc.description.abstractDiabetic nephropathy (DN) is a major complication of diabetic patients and the leading cause of end-stage renal disease. Glomerular dysfunction plays a critical role in DN, but deterioration of renal function also correlates with tubular alterations. Human DN is characterized by glycogen accumulation in tubules. Although this pathological feature has long been recognized, little information exists about the triggering mechanism. In this study, we detected over-expression of muscle glycogen synthase (MGS) in diabetic human kidney. This enhanced expression suggests the participation of MGS in renal metabolic changes associated with diabetes. HK2 human renal cell line exhibited an intrinsic ability to synthesize glycogen, which was enhanced after over-expression of protein targeting to glycogen. A correlation between increased glycogen amount and cell death was observed. Based on a previous transcriptome study on human diabetic kidney disease, significant differences in the expression of genes involved in glycogen metabolism were analyzed. We propose that glucose, but not insulin, is the main modulator of MGS activity in HK2 cells, suggesting that blood glucose control is the best approach to modulate renal glycogen-induced damage during long-term diabetes.
dc.description.funderFondo Nacional de Desarrollo Cientifico y Tecnologico from Chilean State
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00418-014-1290-2
dc.identifier.eissn1432-119X
dc.identifier.issn0948-6143
dc.identifier.urihttps://doi.org/10.1007/s00418-014-1290-2
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101588
dc.identifier.wosidWOS:000349246200008
dc.issue.numero3
dc.language.isoen
dc.pagina.final324
dc.pagina.inicio313
dc.revistaHistochemistry and cell biology
dc.rightsacceso restringido
dc.subjectGlycogen
dc.subjectGlycogen synthase
dc.subjectDiabetic nephropathy
dc.subjectHuman kidney
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleOver-expression of muscle glycogen synthase in human diabetic nephropathy
dc.typeartículo
dc.volumen143
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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