The cotranscribed <i>Salmonella enterica</i> sv. Typhi <i>tsx</i> and <i>impX</i> genes encode opposing nucleoside-specific import and export proteins

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dc.contributor.authorBucarey, Sergio A.
dc.contributor.authorVillagra, Nicolas A.
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorMora, Guido C.
dc.date.accessioned2025-01-21T01:06:12Z
dc.date.available2025-01-21T01:06:12Z
dc.date.issued2006
dc.description.abstractThe Salmonella, enterica tsx gene encodes a nucleoside-specific outer membrane channel. The Tsx porin is essential for the prototrophic growth of S. enterica sv. Typhi in the absence of nucleosides. RT-PCR analysis shows that the tsx gene is cotranscribed with an open reading frame unique to S. enterica, impX (STY0450), which encodes an inner membrane protein 108 amino acids in length, which is predicted to have only two transmembrane a-helices. Fusions of the lacZgene to both tsx and impX reveal that the transcription of both genes is induced in the presence of adenosine. A null mutation in the S. Typhi impX gene suppresses the induced auxotrophy for adenosine or thymidine resulting from a tsx mutation and confers sensitivity to high concentrations of adenosine or thymidine. The ImpX protein, when tagged with a 3xFLAG epitope, is functional and associates with the inner membrane; impX mutants are defective in the export of H-3-radiolabeled thymidine. Taken together, these and other results suggest that the S. Typhi Tsx porin and ImpX inner membrane protein facilitate competing mechanisms of thymidine influx and efflux, respectively, to maintain the steady state levels of internal nucleoside pools.
dc.fuente.origenWOS
dc.identifier.doi10.1534/genetics.105.054700
dc.identifier.eissn1943-2631
dc.identifier.issn0016-6731
dc.identifier.urihttps://doi.org/10.1534/genetics.105.054700
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96110
dc.identifier.wosidWOS:000237990600003
dc.issue.numero1
dc.language.isoen
dc.pagina.final34
dc.pagina.inicio25
dc.revistaGenetics
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe cotranscribed <i>Salmonella enterica</i> sv. Typhi <i>tsx</i> and <i>impX</i> genes encode opposing nucleoside-specific import and export proteins
dc.typeartículo
dc.volumen173
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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