Formation of a Conducting Polymer by Different Electrochemical Techniques and Their Effect on Obtaining an Immunosensor for Immunoglobulin G

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dc.contributor.authorMartinez-Sade, Erika
dc.contributor.authorMartinez-Rojas, Francisco
dc.contributor.authorRamos, Danilo
dc.contributor.authorAguirre, Maria Jesus
dc.contributor.authorArmijo, Francisco
dc.date.accessioned2025-01-20T20:16:58Z
dc.date.available2025-01-20T20:16:58Z
dc.date.issued2023
dc.description.abstractIn this work, a conducting polymer (CP) was obtained through three electrochemical procedures to study its effect on the development of an electrochemical immunosensor for the detection of immunoglobulin G (IgG-Ag) by square wave voltammetry (SWV). The glassy carbon electrode modified with poly indol-6-carboxylic acid (6-PICA) applied the cyclic voltammetry technique presented a more homogeneous size distribution of nanowires with greater adherence allowing the direct immobilization of the antibodies (IgG-Ab) to detect the biomarker IgG-Ag. Additionally, 6-PICA presents the most stable and reproducible electrochemical response used as an analytical signal for developing a label-free electrochemical immunosensor. The different steps in obtaining the electrochemical immunosensor were characterized by FESEM, FTIR, cyclic voltammetry, electrochemical impedance spectroscopy, and SWV. Optimal conditions to improve performance, stability, and reproducibility in the immunosensing platform were achieved. The prepared immunosensor has a linear detection range of 2.0-16.0 ng center dot mL(-1) with a low detection limit of 0.8 ng center dot mL(-1). The immunosensing platform performance depends on the orientation of the IgG-Ab, favoring the formation of the immuno-complex with an affinity constant (Ka) of 4.32 x 10(9) M-1, which has great potential to be used as point of care testing (POCT) device for the rapid detection of biomarkers.
dc.fuente.origenWOS
dc.identifier.doi10.3390/polym15051168
dc.identifier.eissn2073-4360
dc.identifier.urihttps://doi.org/10.3390/polym15051168
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92366
dc.identifier.wosidWOS:000948233200001
dc.issue.numero5
dc.language.isoen
dc.revistaPolymers
dc.rightsacceso restringido
dc.subjectnanostructure
dc.subjectconducting polymer
dc.subjectimmunoglobulin G
dc.subjectelectrochemical immunosensor
dc.subjectsquare wave voltammetry
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFormation of a Conducting Polymer by Different Electrochemical Techniques and Their Effect on Obtaining an Immunosensor for Immunoglobulin G
dc.typeartículo
dc.volumen15
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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