Non-canonical Wnt Signaling Induces Ubiquitination and Degradation of Syndecan

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dc.contributor.authorCarvallo, Loreto
dc.contributor.authorMunoz, Rosana
dc.contributor.authorBustos, Francisco
dc.contributor.authorEscobedo, Noelia
dc.contributor.authorCarrasco, Hector
dc.contributor.authorOlivares, Gonzalo
dc.contributor.authorLarrain, Juan
dc.date.accessioned2024-01-10T12:40:54Z
dc.date.available2024-01-10T12:40:54Z
dc.date.issued2010
dc.description.abstractDynamic regulation of cell adhesion receptors is required for proper cell migration in embryogenesis, tissue repair, and cancer. Integrins and Syndecan4 (SDC4) are the main cell adhesion receptors involved in focal adhesion formation and are required for cell migration. SDC4 interacts biochemically and functionally with components of the Wnt pathway such as Frizzled7 and Dishevelled. Non-canonical Wnt signaling, particularly components of the planar cell polarity branch, controls cell adhesion and migration in embryogenesis and metastasic events. Here, we evaluate the effect of this pathway on SDC4. We have found that Wnt5a reduces cell surface levels and promotes ubiquitination and degradation of SDC4 in cell lines and dorsal mesodermal cells from Xenopus gastrulae. Gain-and loss-of-function experiments demonstrate that Dsh plays a key role in regulating SDC4 steady-state levels. Moreover, a SDC4 deletion construct that interacts inefficiently with Dsh is resistant to Wnt5a-induced degradation. Non-canonical Wnt signaling promotes monoubiquitination of the variable region of SDC4 cytoplasmic domain. Mutation of these specific residues abrogates ubiquitination and results in increased SDC4 steady-state levels. This is the first example of a cell surface protein ubiquitinated and degraded in a Wnt/Dsh-dependent manner.
dc.fechaingreso.objetodigital2024-04-25
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1074/jbc.M110.155812
dc.identifier.eissn1083-351X
dc.identifier.pubmedidMEDLINE:20639201
dc.identifier.urihttps://doi.org/10.1074/jbc.M110.155812
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77360
dc.identifier.wosidWOS:000281740100056
dc.information.autorucCiencias Biológicas; Bustos F;S/I;149780
dc.information.autorucCiencias Biológicas;Carrasco H ;S/I;7258
dc.information.autorucCiencias Biológicas;Carvallo L;S/I;167863
dc.information.autorucCiencias Biológicas;Escobedo N ;S/I;174372
dc.information.autorucCiencias Biológicas;Larrain J ;S/I;90468
dc.information.autorucCiencias Biológicas;Munoz R;S/I;158784
dc.information.autorucCiencias Biológicas;Olivares G ;S/I;13107
dc.issue.numero38
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final29555
dc.pagina.inicio29546
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
dc.revistaJOURNAL OF BIOLOGICAL CHEMISTRY
dc.rightsacceso abierto
dc.subjectPLANAR CELL POLARITY
dc.subjectTYROSINE KINASE ROR2
dc.subjectGASTRULATION MOVEMENTS
dc.subjectMIGRATION
dc.subjectXENOPUS
dc.subjectENDOCYTOSIS
dc.subjectRECEPTOR
dc.subjectADHESION
dc.subjectINTEGRIN
dc.subjectPATHWAY
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNon-canonical Wnt Signaling Induces Ubiquitination and Degradation of Syndecan
dc.typeartículo
dc.volumen285
sipa.codpersvinculados149780
sipa.codpersvinculados7258
sipa.codpersvinculados167863
sipa.codpersvinculados174372
sipa.codpersvinculados90468
sipa.codpersvinculados158784
sipa.codpersvinculados13107
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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