Lysozyme modification by the Fenton reaction and gamma radiation

Simple item page
dc.contributor.authorEdwards, AM
dc.contributor.authorRuiz, M
dc.contributor.authorSilva, E
dc.contributor.authorLissi, E
dc.date.accessioned2024-01-10T12:40:10Z
dc.date.available2024-01-10T12:40:10Z
dc.date.issued2002
dc.description.abstractA comparative study was performed on lysozyme modification after exposure to Fenton reagent (Fe(E)/H2O2) or hydroxyl radicals produced by gamma radiation. The conditions were adjusted to obtain, with both systems, a 50% loss of activity of the modified ensemble. gamma radiation modified almost all types of amino acid residues in the enzyme, with little specificity. The modification order was Tyr >Met = Cys > Lys > Ile + Leu > Gly > Pro = Phe > Thr + Ala > Trp = Ser > Arg > Asp + Glu, with 42 mol of modified residues per initial mole of native enzyme. In contrast, when the enzyme was exposed to the Fenton reaction, only some types of amino acids were modified. Furthermore, a smaller number of residues (13.5) were damaged per initial mole of enzyme. The order of the modified residues was Tyr > Cys > Trp > Met > His > Ile + Leu > Val > Arg. These results demonstrate that the modifications elicited by these two free radical sources follow different mechanisms. An intramolecular free radical chain reaction is proposed to play a dominant role in the oxidative modification of the protein promoted by gamma radiation.
dc.fechaingreso.objetodigital2024-05-09
dc.format.extent8 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1080/10715760290019291
dc.identifier.issn1071-5762
dc.identifier.pubmedidMEDLINE:12071346
dc.identifier.urihttps://doi.org/10.1080/10715760290019291
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77279
dc.identifier.wosidWOS:000174725300006
dc.information.autorucQuímica;Edwards A;S/I;53657
dc.information.autorucQuímica;Silva E;S/I;98771
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final284
dc.pagina.inicio277
dc.publisherTAYLOR & FRANCIS LTD
dc.revistaFREE RADICAL RESEARCH
dc.rightsacceso restringido
dc.subjectlysozyme
dc.subjectgamma radiation
dc.subjectFenton reaction
dc.subjecthydroxyl radical
dc.subjectBIOLOGICALLY RELEVANT
dc.subjectOXYGEN RADICALS
dc.subjectPROTEIN DAMAGE
dc.subjectPEPTIDE-BOND
dc.subjectAMINO-ACIDS
dc.subjectOXIDATION
dc.subjectINACTIVATION
dc.subjectDEGRADATION
dc.subjectPHOTOOXIDATION
dc.subjectTRYPTOPHAN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLysozyme modification by the Fenton reaction and gamma radiation
dc.typeartículo
dc.volumen36
sipa.codpersvinculados53657
sipa.codpersvinculados98771
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2024-05-09. Lysozyme modification by the Fenton reaction and gamma radiation.pdf
Size:
2.69 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas