Human equilibrative nucleoside transporters 1 and 2 may be differentially modulated by A(2B) adenosine receptors in placenta microvascular endothelial cells from pre-eclampsia

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dc.contributor.authorEscudero, C.
dc.contributor.authorCasanello, P.
dc.contributor.authorSobrevia, L.
dc.date.accessioned2024-01-10T12:39:27Z
dc.date.available2024-01-10T12:39:27Z
dc.date.issued2008
dc.description.abstractPre-eclampsia is associated with elevated maternal blood pressure and proteinuria, altered fetal growth, and increased plasma adenosine concentration in the mother and the fetus. Human equilibrative nucleoside transporters 1 (hENT1) and hENT2 are crucial to maintain physiological plasma levels of adenosine, thus modulating its several biological effects through adenosine receptor activation. However, it is unknown whether hENTs and adenosine receptors are expressed in human placental microvascular endothelium (hPMEC). To assay whether the increased fetal plasma adenosine concentration in pre-eclampsia results from altered hENT-mediated transport, and the potential involvement of adenosine receptors in this phenomenon, we investigated hENTs and A(2A) and A(2B) adenosine receptors expression and function in hPMEC. Cells were isolated and Cultured from normal pregnancies (n = 17) or Pre-eclampsia with adequate-for-gestational age fetuses (n = 7). hENT1, hENT2, A(2A) and A(2B) adenosine receptors were expressed and functional in hPMEC. Extracelhilar adenosine concentration was higher (4-fold) in pre-eclampsia versus normal pregnancies. hPMEC from pre-eclampsia exhibit increased total ;transport (hENT1 + hENT2), and maximal velocity (V-max) for hENT2- (2-fold), but reduced V-max for hENT1-mediated adenosine transport (75%), with no changes in apparent K-m. hENT2 expression was increased (4.5-fold), but hENT1 protein abundance was reduced (80%) in pre-eclampsia. Equally, A2A expression was reduced (50-80%) in pre-eclampsia. CGS-21680 (A(2A) agonist) did not alter hENTs expression or activity, but ZM-241385 (A(2A) antagonist) blocked pre-eclampsia effects and increased hENT1-mechated transport in normal pregnancies. Thus, A(2B) adenosine receptors may differentially modulate hENTs in hPMEC, which could be a mechanism attempting to re-establish physiological extracellular adenosine levels in pre-eclampsia. (C) 2008 Elsevier Ltd. All rights reserved.
dc.description.funderFondo Nacional de Ciencia y Tecnologia
dc.description.funderComision Nacional de Investigacion Cientifica y Tecnologica
dc.description.funderVice-Rectoria de Asuntos Academicos e Investigacion (VRAID)
dc.description.funderPontificia Universidad Catolica de Chile
dc.fechaingreso.objetodigital05-04-2024
dc.format.extent10 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.placenta.2008.06.014
dc.identifier.eissn1532-3102
dc.identifier.issn0143-4004
dc.identifier.pubmedidMEDLINE:18703227
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2008.06.014
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77193
dc.identifier.wosidWOS:000259712600008
dc.information.autorucMedicina;Casanello P;S/I;146772
dc.information.autorucMedicina;Escudero C;S/I;158196
dc.information.autorucMedicina;Sobrevia L;S/I;1002656
dc.issue.numero9
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final825
dc.pagina.inicio816
dc.publisherW B SAUNDERS CO LTD
dc.revistaPLACENTA
dc.rightsacceso restringido
dc.subjecthuman
dc.subjectplacenta
dc.subjectadenosine
dc.subjectendothelium
dc.subjectpre-eclampsia
dc.subjectUMBILICAL VEIN ENDOTHELIUM
dc.subjectSLC29A1 PROMOTER ACTIVITY
dc.subjectFETAL-GROWTH RESTRICTION
dc.subjectADENOSINE TRANSPORT
dc.subjectPLASMA ADENOSINE
dc.subjectNUCLEOSIDE
dc.subjectRECEPTORS
dc.subjectPREGNANCY
dc.subjectEXPRESSION
dc.subjectVASOCONSTRICTION
dc.subject.ods03 Good Health and Well-being
dc.subject.ods05 Gender Equality
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa05 Igualdad de género
dc.titleHuman equilibrative nucleoside transporters 1 and 2 may be differentially modulated by A(2B) adenosine receptors in placenta microvascular endothelial cells from pre-eclampsia
dc.typeartículo
dc.volumen29
sipa.codpersvinculados146772
sipa.codpersvinculados158196
sipa.codpersvinculados1002656
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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