Gold@Silica Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering

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dc.contributor.authorJose Marchant, Maria
dc.contributor.authorGuzman, Leda
dc.contributor.authorCorvalan, Alejandro H.
dc.contributor.authorKogan, Marcelo J.
dc.date.accessioned2025-01-23T21:10:49Z
dc.date.available2025-01-23T21:10:49Z
dc.date.issued2019
dc.description.abstractReprimo (RPRM) is a tumor suppressor gene involved in the development of gastric cancer. Hypermethylation of the RPRM promoter region has been found in tumor tissue and plasma samples from patients with gastric cancer. These findings suggest that circulating methylated DNA of RPRM could be a candidate for a noninvasive detection of gastric cancer. We designed a nanosystem based on the functionalization of silica coated gold nanoparticles with oligonucleotides that recognize a specific DNA fragment of the RPRM promoter region. The functionality of the oligonucleotide on the surface of the nanoparticle was confirmed by polymerase chain reaction (PCR). The nanoparticles were incubated with a synthetic DNA fragment of methylated DNA of RPRM and changes in the size distribution after hybridization were evaluated by dynamic light scattering (DLS). A difference in the size distribution of nanoparticles hybridized with genomic DNA from the KATO III gastric cancer cell line was observed when was compared with DNA from the GES-1 normal cell line. These results showed that this nanosystem may be a useful tool for the specific and sensitive detection of methylated DNA of RPRM in patients at risk of developing gastric cancer.
dc.fuente.origenWOS
dc.identifier.doi10.3390/nano9091333
dc.identifier.eissn2079-4991
dc.identifier.urihttps://doi.org/10.3390/nano9091333
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100863
dc.identifier.wosidWOS:000489101900150
dc.issue.numero9
dc.language.isoen
dc.revistaNanomaterials
dc.rightsacceso restringido
dc.subjectgold nanoparticles
dc.subjectmethylated RPRM DNA
dc.subjectdynamic light scattering
dc.subjectgastric cancer
dc.subjectnoninvasive detection
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGold@Silica Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering
dc.typeartículo
dc.volumen9
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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