High-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

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dc.contributor.authorDettleff, Phillip
dc.contributor.authorZuloaga, Rodrigo
dc.contributor.authorFuentes, Marcia
dc.contributor.authorGonzalez, Pamela
dc.contributor.authorAedo, Jorge
dc.contributor.authorManuel Estrada, Juan
dc.contributor.authorMolina, Alfredo
dc.contributor.authorAntonio Valdes, Juan
dc.date.accessioned2024-01-10T13:45:20Z
dc.date.available2024-01-10T13:45:20Z
dc.date.issued2022
dc.description.abstractSimple Summary The red cusk-eel (Genypterus chilensis) is a native Chilean species important for aquaculture diversification in Chile. The effect of high-temperature stress on the liver, a key organ for fish metabolism, is unknown. In this study we determined for the first time the effects of high-temperature stress on the liver of red cusk-eel. The results showed that high-temperature stress increased hepatic enzyme activity in the plasma of stressed fish. Additionally, this stressor generated oxidative damage in liver, and generated a transcriptional response with 1239 down-regulated and 1339 up-regulated transcripts associated with several processes, including unfolded protein response, heat shock response and oxidative stress, among others. Together, these results indicate that high-temperature stress generates a relevant impact on liver, with should be considered for the aquaculture and fisheries industry of this species under a climate change scenario. Environmental stressors, such as temperature, are relevant factors that could generate a negative effect on several tissues in fish. A key fish species for Chilean aquaculture diversification is the red cusk-eel (Genypterus chilensis), a native fish for which knowledge on environmental stressors effects is limited. This study evaluated the effects of high-temperature stress on the liver of red cusk-eel in control (14 degrees C) and high-temperature (19 degrees C) groups using multiple approaches: determination of plasmatic hepatic enzymes (ALT, AST, and AP), oxidative damage evaluation (AP sites, lipid peroxidation, and carbonylated proteins), and RNA-seq analysis. High-temperature stress generated a significant increase in hepatic enzyme activity in plasma. In the liver, a transcriptional regulation was observed, with 1239 down-regulated and 1339 up-regulated transcripts. Additionally, high-temperature stress generated oxidative stress in the liver, with oxidative damage and transcriptional modulation of the antioxidant response. Furthermore, an unfolded protein response was observed, with several pathways enriched, as well as a heat shock response, with several heat shock proteins up regulated, suggesting candidate biomarkers (i.e., serpinh1) for thermal stress evaluation in this species. The present study shows that high-temperature stress generated a major effect on the liver of red cusk-eel, knowledge to consider for the aquaculture and fisheries of this species.
dc.fechaingreso.objetodigital2024-05-23
dc.fuente.origenWOS
dc.identifier.doi10.3390/biology11070990
dc.identifier.eissn2079-7737
dc.identifier.urihttps://doi.org/10.3390/biology11070990
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79018
dc.identifier.wosidWOS:000832219000001
dc.information.autorucInterdisciplinarias; Dettleff Faundes, Phillip James; S/I; 1252622
dc.issue.numero7
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherMDPI
dc.revistaBIOLOGY-BASEL
dc.rightsacceso abierto
dc.subjectred cusk-eel
dc.subjectthermal stress
dc.subjectRNA-seq
dc.subjectliver transcriptome
dc.subjectoxidative damage
dc.subjectprotein folding
dc.subjecthepatic enzymes
dc.subject.ods14 Life Below Water
dc.subject.odspa14 Vida submarina
dc.titleHigh-Temperature Stress Effect on the Red Cusk-Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage
dc.typeartículo
dc.volumen11
sipa.codpersvinculados1252622
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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