FOXO1 regulates wound-healing responses in human gingival fibroblasts

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dc.contributor.authorRojas, Leticia
dc.contributor.authorTobar, Nicolas
dc.contributor.authorEspinoza, Javier
dc.contributor.authorRios, Susana
dc.contributor.authorMartinez, Constanza
dc.contributor.authorMartinez, Jorge
dc.contributor.authorGraves, Dana T.
dc.contributor.authorSmith, Patricio C.
dc.date.accessioned2025-01-20T17:07:55Z
dc.date.available2025-01-20T17:07:55Z
dc.date.issued2024
dc.description.abstractBackground and objective: Forkhead box-O 1 (FOXO1) is a transcription factor actively involved in oral wound healing at the epithelial barrier. However, less is known regarding the role of FOXO1 during the tissue repair response in the connective tissue compartment. This study explored the involvement of FOXO1 in the modulation of fibroblast activity related to wound healing. Methods: Primary cultures of human gingival fibroblasts were obtained from four healthy young donors. Myofibroblastic differentiation, collagen gel contraction, cell migration, cell spreading, and integrin activation were evaluated in the presence or absence of a FOXO1 inhibitor (AS1842856). Variations in mRNA and proteins of interest were evaluated through qRT-PCR and western blot, respectively. Distribution of actin, alpha-smooth muscle actin, and beta 1 integrin was evaluated using immunofluorescence. FOXO1 and TGF-beta 1 expression in gingival wound healing was assessed by immunohistochemistry in gingival wounds performed in C57BL/6 mice. Images were analyzed using ImageJ/Fiji. ANOVA or Kruskal-Wallis test followed by Tukey's or Dunn's post-hoc test was performed. All data are expressed as mean +/- SD. p < .05 was considered statistically significant. Results: FOXO1 inhibition caused a decrease in the expression of the myofibroblastic marker alpha-SMA along with a reduction in fibronectin, type I collagen, TGF-beta 1, and beta 1 integrin mRNA level. The FOXO1 inhibitor also caused decreases in cell migration, cell spreading, collagen gel contraction, and beta 1 integrin activation. FOXO1 and TGF-beta 1 were prominently expressed in gingival wounds in fibroblastic cells located at the wound bed. Conclusion: The present study indicates that FOXO1 plays an important role in the modulation of several wound-healing functions in gingival fibroblast. Moreover, our findings reveal an important regulatory role for FOXO1 on the differentiation of gingival myofibroblasts, the regulation of cell migration, and collagen contraction, all these functions being critical during tissue repair and fibrosis.
dc.fuente.origenWOS
dc.identifier.doi10.1111/jre.13257
dc.identifier.eissn1600-0765
dc.identifier.issn0022-3484
dc.identifier.urihttps://doi.org/10.1111/jre.13257
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90908
dc.identifier.wosidWOS:001186804800001
dc.issue.numero3
dc.language.isoen
dc.pagina.final621
dc.pagina.inicio611
dc.revistaJournal of periodontal research
dc.rightsacceso restringido
dc.subjectcollagen
dc.subjectconnective tissue
dc.subjectintegrins
dc.subjecttranscription factors
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFOXO1 regulates wound-healing responses in human gingival fibroblasts
dc.typeartículo
dc.volumen59
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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