Fecal bile acid excretion and messenger RNA expression levels of ileal transporters in high risk gallstone patients

Simple item page
dc.contributor.authorHerrera Sepúlveda, Jorge Gabriel
dc.contributor.authorAmigo Boker, Ludwig Peter
dc.contributor.authorBenítez, Carlos
dc.contributor.authorZanlungo Matsuhiro, Silvana
dc.contributor.authorMiquel P., Juan Francisco
dc.contributor.authorNervi, Flavio
dc.contributor.authorHusche, Constanze
dc.contributor.authorLütjohann, Dieter
dc.date.accessioned2019-10-17T15:15:10Z
dc.date.available2019-10-17T15:15:10Z
dc.date.issued2009
dc.date.updated2019-10-14T18:43:32Z
dc.description.abstractAbstract Background Cholesterol gallstone disease (GS) is highly prevalent among Hispanics and American Indians. In GS, the pool of bile acids (BA) is decreased, suggesting that BA absorption is impaired. In Caucasian GS patients, mRNA levels for ileal BA transporters are decreased. We aimed to determine fecal BA excretion rates, mRNA levels for ileal BA transporter genes and of regulatory genes of BA synthesis in Hispanic GS patients. Results Excretion of fecal BA was measured in seven GS females and in ten GS-free individuals, all with a body mass index < 29. Participants ingested the stool marker Cr2O3 (300 mg/day) for 10 days, and fecal specimens were collected on the last 3 days. Chromium was measured by a colorimetric method, and BA was quantitated by gas chromatography/mass spectroscopy. Intake of calories, nutrients, fiber and cholesterol were similar in the GS and GS-free subjects. Mean BA excretion levels were 520 ± 80 mg/day for the GS-free group, and 461 ± 105 mg/day for the GS group. Messenger RNA expression levels were determined by RT-PCR on biopsy samples obtained from ileum during diagnostic colonoscopy (14 GS-free controls and 16 GS patients) and from liver during surgery performed at 8 and 10 AM (12 GS and 10 GS-free patients operated on for gastrointestinal malignancies), all with a body mass index < 29. Messenger RNA level of the BA transporter genes for ileal lipid binding protein, multidrug resistance-associated protein 3, organic solute transporter alpha, and organic solute transporter beta were similar in GS and GS-free subjects. Messenger RNA level of Cyp27A1, encoding the enzyme 27α-hydroxylase, the short heterodimer partner and farnesoid X receptor remained unchanged, whereas the mRNA level of Cyp7A1, the rate limiting step of BA synthesis, was increased more than 400% (p < 0.01) in the liver of GS compared to GS-free subjects. Conclusion Hispanics with GS have fecal BA excretion rates and mRNA levels of genes for ileal BA transporters that are similar to GS-free subjects. However, mRNA expression levels of Cyp7A1 are increased in GS, indicating that regulation of BA synthesis is abnormal in Hispanics with GS.
dc.fuente.origenBiomed Central
dc.identifier.citationLipids in Health and Disease. 2009 Dec 08;8(1):53
dc.identifier.doi10.1186/1476-511X-8-53
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/26758
dc.identifier.wosidWOS:000273122100001
dc.issue.numeroNo. 53
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final6
dc.pagina.inicio1
dc.revistaLipids in Health and Disease volumees_ES
dc.rightsacceso abierto
dc.rights.holderHerrera et al; licensee BioMed Central Ltd.
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.subject.otherColelitiasises_ES
dc.subject.otherCálculos biliareses_ES
dc.subject.otherColesteroles_ES
dc.titleFecal bile acid excretion and messenger RNA expression levels of ileal transporters in high risk gallstone patientses_ES
dc.typeartículo
dc.volumenVol. 8
sipa.codpersvinculados9202
sipa.codpersvinculados72650
sipa.codpersvinculados72216
sipa.codpersvinculados99156
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12944_2009_Article_241.pdf
Size:
239.73 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos de revistas