Microbial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods

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dc.contributor.authorDiGiulio, Daniel B.
dc.contributor.authorGervasi, MariaTeresa
dc.contributor.authorRomero, Roberto
dc.contributor.authorMazaki Tovi, Shali
dc.contributor.authorVaisbuch, Edi
dc.contributor.authorKusanovic, Juan Pedro
dc.contributor.authorSeok, Kimberley S.
dc.contributor.authorGomez, Ricardo
dc.contributor.authorMittal, Pooja
dc.contributor.authorGotsch, Francesca
dc.contributor.authorChaiworapongsa, Tinnakorn
dc.contributor.authorOyarzun, Enrique
dc.contributor.authorKim, Chong Jai
dc.contributor.authorRelman, David A.
dc.date.accessioned2024-01-10T12:06:37Z
dc.date.available2024-01-10T12:06:37Z
dc.date.issued2010
dc.description.abstractObjective: Infection has been implicated in the pathogenesis of preeclampsia, yet the association between microbial invasion of the amniotic cavity (MIAC) and preeclampsia has not been determined. The aim of this study was to determine the prevalence, and microbial diversity associated with MIAC, as well as the nature of the host response to MIAC in patients with preeclampsia.
dc.description.abstractMethod of study: Amniotic fluid (AF) from 62 subjects with preeclampsia, not in labor, was analyzed with both cultivation and molecular methods. Broad-range and group-specific PCR assays targeting small subunit ribosomal DNA, or other gene sequences, from bacteria, fungi and archaea were used. Results were correlated with measurements of host inflammatory response, including AF white blood cell count and AF concentrations of glucose, interleukin-6 (IL-6) and MMP-8.
dc.description.abstractResults: 1) The rate of MIAC in preeclampsia was 1.6% (1/62) based on cultivation techniques, 8% (5/62) based on PCR, and 9.6% (6/62) based on the combined results of both methods; 2) among the six patients diagnosed with MIAC, three had a positive PCR for Sneathia/Leptotrichia spp.; and 3) patients with MIAC were more likely to have evidence of an inflammatory response in the amniotic cavity than those without MIAC, as determined by a higher median AF IL-6 [1.65 ng/mL interquartile range (IQR): 0.35-4.62 vs. 0.22 ng/mL IQR: 0.12-0.51; P=0.002).
dc.description.abstractConclusion: The prevalence of MIAC in preeclampsia is low, suggesting that intra-amniotic infection plays only a limited role in preeclampsia. However, the unexpectedly high number of positive AF specimens for Sneathia/Leptotrichia warrants further investigation.
dc.description.funderEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, DHHS
dc.description.funderMarch of Dimes Foundation
dc.description.funderNIH
dc.description.funderThomas C. and Joan M. Merigan Endowment at Stanford University
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
dc.description.funderEUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT
dc.description.funderOFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1515/JPM.2010.078
dc.identifier.eissn1619-3997
dc.identifier.issn0300-5577
dc.identifier.pubmedidMEDLINE:20482470
dc.identifier.urihttps://doi.org/10.1515/JPM.2010.078
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76186
dc.identifier.wosidWOS:000281566600009
dc.information.autorucMedicina; Oyarzun E;S/I;54261
dc.information.autorucMedicina;Gomez R;S/I;80926
dc.issue.numero5
dc.language.isoen
dc.nota.accesoSin adjunto
dc.pagina.final513
dc.pagina.inicio503
dc.publisherWALTER DE GRUYTER GMBH
dc.revistaJOURNAL OF PERINATAL MEDICINE
dc.rightsregistro bibliográfico
dc.subjectIL-6
dc.subjectintra-amniotic infection
dc.subjectintra-amniotic inflammation
dc.subjectPCR
dc.subjectpreeclampsia
dc.subjectpregnancy
dc.subject16S rRNA
dc.subjectSneathia/Leptotrichia spp.
dc.subjectUreaplasma urealyticum
dc.subjectENDOTHELIAL GROWTH-FACTOR
dc.subjectFOR-GESTATIONAL-AGE
dc.subjectTUMOR-NECROSIS-FACTOR
dc.subjectCIRCULATING ANGIOGENIC FACTORS
dc.subjectELEVATED LIVER-ENZYMES
dc.subjectREAL-TIME PCR
dc.subjectNORMAL-PREGNANCY
dc.subjectOXIDATIVE STRESS
dc.subjectMATERNAL PLASMA
dc.subjectFACTOR RECEPTOR-1
dc.subject.ods03 Good Health and Well-being
dc.subject.ods05 Gender Equality
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa05 Igualdad de género
dc.titleMicrobial invasion of the amniotic cavity in preeclampsia as assessed by cultivation and sequence-based methods
dc.typeartículo
dc.volumen38
sipa.codpersvinculados54261
sipa.codpersvinculados80926
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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