Differential levels of anti-Mycobacterium tuberculosis-specific IgAs in saliva of household contacts with latent tuberculosis infection

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dc.article.number1267670
dc.catalogadorvzp
dc.contributor.authorRuiz-Tagle Seguel, Cinthya Grace
dc.contributor.authorNaves Pichuante, Rodrigo Antonio
dc.contributor.authorGarcia Canete, Patricia Del Carmen
dc.contributor.authorGuenther, Anna
dc.contributor.authorSchneiderhan-Marra, Nicole
dc.contributor.authorBalcells Marty, Maria Elvira
dc.date.accessioned2023-11-10T16:07:52Z
dc.date.available2023-11-10T16:07:52Z
dc.date.issued2023
dc.description.abstractIntroduction: Mucosal immunity is strongly elicited in early stages of many respiratory and enteric infections; however, its role in tuberculosis pathogenesis has been scarcely explored. We aimed to investigate Mycobacterium tuberculosis (Mtb) specific IgA levels in saliva in different stages of latent Tuberculosis Infection (TBI).Methodology: A multiplex bead-based Luminex immunoassay was developed to detect specific IgA against 12 highly immunogenic Mtb antigens. A prospective cohort of household contacts (>14 years) of pulmonary TB cases was established in Santiago, Chile. Contacts were classified as Mtb-infected or not depending on serial interferon-gamma release assay results. Saliva samples were collected and tested at baseline and at a 12-week follow-up.Results: Mtb-specific IgA was detectable at all visits in all participants (n = 168), including the "non-Mtb infected" (n = 64). Significantly higher median levels of IgA were found in the "Mtb infected" compared to the uninfected for anti-lipoarabinomannan (LAM) (110 vs. 84.8 arbitrary units (AU), p < 0.001), anti-PstS1 (117 vs. 83 AU, p < 0.001), anti-Cell Membrane Fraction (CMF) (140 vs. 103 AU, p < 0.001) and anti-Culture Filtrate Proteins (CFP) (median 125 vs. 96 AU, p < 0.001), respectively. Nonetheless, the discriminatory performance of these specific mucosal IgA for TBI diagnosis was low.Conclusion: Saliva holds Mtb-specific IgA against several antigens with increased levels for anti-LAM, anti-PstS1, anti-CMF and anti-CFP found in household contacts with an established TBI. The role of these mucosal antibodies in TB pathogenesis, and their kinetics in different stages of Mtb infection merits further exploring.
dc.fechaingreso.objetodigital2023-11-10
dc.format.extent13 páginas
dc.fuente.origenWOS
dc.identifier.doi10.3389/fmed.2023.1267670
dc.identifier.eissn2296-858X
dc.identifier.urihttps://doi.org/10.3389/fmed.2023.1267670
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75276
dc.identifier.wosidWOS:001085965100001
dc.information.autorucEscuela de Medicina; Ruiz-tagle Seguel Cinthya Grace; 0000-0002-4712-3302; 1098594
dc.information.autorucEscuela de Medicina; Naves Pichuante Rodrigo Antonio; 0000-0002-1203-3593; 200771
dc.information.autorucEscuela de Medicina; Garcia Canete Patricia Del Carmen; 0000-0002-5983-2392; 73909
dc.information.autorucEscuela de Medicina; Balcells Marty Maria Elvira; 0000-0002-7223-9665; 7462
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final13
dc.pagina.inicio01
dc.publisherFRONTIERS MEDIA SA
dc.revistaFRONTIERS IN MEDICINE
dc.rightsacceso restringido
dc.subjecttuberculosis
dc.subjectsaliva
dc.subjectimmunoglobulin
dc.subjectIgA
dc.subjectantibody
dc.subjectbiomarker
dc.subjectdiagnostic test
dc.subjectMycobacterium tuberculosis
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDifferential levels of anti-Mycobacterium tuberculosis-specific IgAs in saliva of household contacts with latent tuberculosis infection
dc.typeartículo
dc.volumen10
sipa.codpersvinculados1098594
sipa.codpersvinculados200771
sipa.codpersvinculados73909
sipa.codpersvinculados7462
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