The <i>Salmonella</i> Typhi <i>hlyE</i> gene plays a role in invasion of cultured epithelial cells and its functional transfer to <i>S.</i> Typhimurium promotes deep organ infection in mice

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dc.contributor.authorFuentes, Juan A.
dc.contributor.authorVillagra, Nicolas
dc.contributor.authorCastillo-Ruiz, Mario
dc.contributor.authorMora, Guido C.
dc.date.accessioned2025-01-21T01:05:06Z
dc.date.available2025-01-21T01:05:06Z
dc.date.issued2008
dc.description.abstractComparison of genome sequences of Salmonella enterica serovars Typhi and Typhimurium reveals that S. Typhi has a small 2.3 kb genomic island missing in S. Typhimurium, designated Salmonella pathogenicity island 18 (SPI-18), which includes two potential genes. One of these, hlyE, encodes a hemolysin related to the Escherichia coli K12 HlyE hemolysin. PCR assays show that SPI-18 is present in S. Typhi and in many other, but not all, serovars of S. enterica subsp. enterica belonging to the SARB collection. HlyE activity cannot be detected in S. Typhi by means of standard plate assays. Nevertheless, we were able to reveal this activity upon lysis of bacterial cells with phages, in the presence of ampicillin, and in a ompA genetic background, conditions that compromise the integrity of the bacterial envelope. Almost all serovars of the SARB collection shown to cause systemic infections in humans have SPI-18 and hlyE and express an active hemolysin revealed upon bacterial envelope destabilization. S. Typhi hlyE mutants are impaired in invasion of human epithelial cells in vitro, and its heterologous expression in S. Typhimurium improves the colonization of deep organs in mice, demonstrating that the HlyE hemolysin is a new virulence determinant. (C) 2008 Published by Elsevier Masson SAS.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.resmic.2008.02.006
dc.identifier.eissn1769-7123
dc.identifier.issn0923-2508
dc.identifier.urihttps://doi.org/10.1016/j.resmic.2008.02.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95815
dc.identifier.wosidWOS:000257902100008
dc.issue.numero4
dc.language.isoen
dc.pagina.final287
dc.pagina.inicio279
dc.revistaResearch in microbiology
dc.rightsacceso restringido
dc.subjectSalmonella typhi
dc.subjectinvasion
dc.subjectHlyE
dc.subjecthemolysin
dc.subjectSPI-18
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe <i>Salmonella</i> Typhi <i>hlyE</i> gene plays a role in invasion of cultured epithelial cells and its functional transfer to <i>S.</i> Typhimurium promotes deep organ infection in mice
dc.typeartículo
dc.volumen159
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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