Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of <i>Argylia radiata</i> Vitroplants and Natural Roots

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dc.contributor.authorGiordano, Ady
dc.contributor.authorMorales-Tapia, Pablo
dc.contributor.authorMoncada-Basualto, Mauricio
dc.contributor.authorPozo-Martinez, Josue
dc.contributor.authorOlea-Azar, Claudio
dc.contributor.authorNesic, Aleksandra
dc.contributor.authorCabrera-Barjas, Gustavo
dc.date.accessioned2025-01-20T21:13:38Z
dc.date.available2025-01-20T21:13:38Z
dc.date.issued2022
dc.description.abstractPlant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25-68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 mu g/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.
dc.fuente.origenWOS
dc.identifier.doi10.3390/molecules27030610
dc.identifier.eissn1420-3049
dc.identifier.urihttps://doi.org/10.3390/molecules27030610
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93714
dc.identifier.wosidWOS:000754808300001
dc.issue.numero3
dc.language.isoen
dc.revistaMolecules
dc.rightsacceso restringido
dc.subjectArgylia radiata
dc.subjectvitroplants
dc.subjectpolyphenolic extracts
dc.subjectantioxidant activity
dc.subjectORAC
dc.subjectelectronic paramagnetic resonance (EPR)
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePolyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of <i>Argylia radiata</i> Vitroplants and Natural Roots
dc.typeartículo
dc.volumen27
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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