Gene expression studies in isolated mitochondria

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dc.contributor.authorChoury, D
dc.contributor.authorFarré, JC
dc.contributor.authorJordana, X
dc.contributor.authorAraya, A
dc.date.accessioned2025-01-21T01:07:15Z
dc.date.available2025-01-21T01:07:15Z
dc.date.issued2005
dc.description.abstractThe complex gene expression mechanisms that occur in plant mitochondria, such as RNA editing and splicing, are not yet well understood. RNA editing in higher plant mitochondria is a highly specific process which modifies mRNA sequences by C-to-U conversions. It has been suggested that in some cases this process is required for splicing. Here, we use an experimental model based on the introduction of DNA into isolated mitochondria by electroporation to study organellar gene expression events. Our aim was to compare processing and editing of potato small ribosomal protein 10 gene (rps10) transcripts in heterologous (wheat mitochondria) and homologous (potato mitochondria) contexts. rps10 is a suitable model because it contains a group II intron, is absent in wheat mitochondria but is actively expressed in potato mitochondria, where transcripts are spliced and undergo five C-to-U editing events. For this purpose, conditions for electroporating isolated potato mitochondria were established. rps10 was placed under the control of either potato or wheat cox2 promoters. We found that rps10 was only transcribed under the control of a cognate promoter. In wheat mitochondria, rps10 transcripts were neither spliced nor edited while they are correctly processed in potato mitochondria. Interestingly, a wheat editing site grafted into rps10 was not recognized by wheat mitochondria but was correctly edited in potato mitochondria. Taken together, these results suggest that editing might occur only when the transcripts are engaged in processing and that they would not be available to editing factors outside of a putative RNA maturation machinery complex.
dc.fuente.origenWOS
dc.identifier.doi10.1093/nar/gki1017
dc.identifier.eissn1362-4962
dc.identifier.issn0305-1048
dc.identifier.urihttps://doi.org/10.1093/nar/gki1017
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96299
dc.identifier.wosidWOS:000234436200021
dc.issue.numero22
dc.language.isoen
dc.pagina.final7065
dc.pagina.inicio7058
dc.revistaNucleic acids research
dc.rightsacceso restringido
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleGene expression studies in isolated mitochondria
dc.typeartículo
dc.volumen33
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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