Co-Infection by LF-89-Like and EM-90-Like Genogroups of Piscirickettsia Salmonis in Farmed Atlantic Salmon in Chile: Implications for Surveillance and Control of Piscirickettsiosis

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dc.catalogadorjwg
dc.contributor.authorRozas-Serri, Marco
dc.contributor.authorPena, Andrea
dc.contributor.authorGardner, Ian
dc.contributor.authorPenaloza, Estefania
dc.contributor.authorMaldonado, Lucerina
dc.contributor.authorMunoz, Ariel
dc.contributor.authorMardones Loyola, Fernando Otoniel
dc.contributor.authorRodriguez, Catalina
dc.contributor.authorIldefonso, Ricardo
dc.contributor.authorSenn, Carolina
dc.contributor.authorAranis, Felipe
dc.date.accessioned2024-01-25T20:17:59Z
dc.date.available2024-01-25T20:17:59Z
dc.date.issued2023
dc.description.abstractPiscirickettsiosis (SRS), caused by Piscirickettsia salmonis, is the main infectious disease that affects farmed Atlantic salmon in Chile. Currently, the official surveillance and control plan for SRS in Chile is based only on the detection of P. salmonis, but neither of its genogroups (LF-89-like and EM-90-like) are included. Surveillance at the genogroup level is essential not only for defining and evaluating the vaccination strategy against SRS, but it is also of utmost importance for early diagnosis, clinical prognosis in the field, treatment, and control of the disease. The objectives of this study were to characterize the spatio-temporal distribution of P. salmonis genogroups using genogroup-specific real-time probe-based polymerase chain reaction (qPCR) to discriminate between LF-89-like and EM-90-like within and between seawater farms, individual fish, and tissues/organs during early infection in Atlantic salmon under field conditions. The spatio-temporal distribution of LF-89-like and EM-90-like was shown to be highly variable within and between seawater farms. P. salmonis infection was also proven to be caused by both genogroups at farm, fish, and tissue levels. Our study demonstrated for the first time a complex co-infection by P. salmonis LF-89-like and EM-90-like in Atlantic salmon. Liver nodules (moderate and severe) were strongly associated with EM-90-like infection, but this phenotype was not detected by infection with LF-89-like or co-infection of both genogroups. The detection rate of P. salmonis LF-89-like increased significantly between 2017 and 2021 and was the most prevalent genogroup in Chilean salmon aquaculture during this period. Lastly, a novel strategy to identify P. salmonis genogroups based on novel genogroup-specific qPCR for LF-89-like and EM-90-like genogroups is suggested.
dc.fechaingreso.objetodigital2024-06-05
dc.fuente.origenWOS
dc.identifier.doi10.3390/pathogens12030450
dc.identifier.eissn2076-0817
dc.identifier.urihttps://doi.org/10.3390/pathogens12030450
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/80955
dc.identifier.wosidWOS:000957861100001
dc.information.autorucEscuela de Medicina; Mardones Loyola Fernando Otoniel; 0000-0003-1228-5392; 1084534
dc.issue.numero3
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherMDPI
dc.revistaPATHOGENS
dc.rightsacceso abierto
dc.subjectAtlantic salmon
dc.subjectPiscirickettsia salmonis
dc.subjectLF-89-like
dc.subjectEM-90-like
dc.subjectCo-infection
dc.subject.ddc600
dc.subject.deweyTecnologíaes_ES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCo-Infection by LF-89-Like and EM-90-Like Genogroups of Piscirickettsia Salmonis in Farmed Atlantic Salmon in Chile: Implications for Surveillance and Control of Piscirickettsiosis
dc.typeartículo
dc.volumen12
sipa.codpersvinculados1084534
sipa.trazabilidadORCID;2024-01-15
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