Inherent differences in nasal and tracheal ciliary function in response to Pseudomonas aeruginosa challenge

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dc.contributor.authorZhao, Ke Qing
dc.contributor.authorGoldstein, Natalia
dc.contributor.authorYang, Haibin
dc.contributor.authorCowan, Andrew T.
dc.contributor.authorChen, Bei
dc.contributor.authorZheng, Chunquan
dc.contributor.authorPalmer, James N.
dc.contributor.authorKreindler, James L.
dc.contributor.authorCohen, Noam A.
dc.date.accessioned2024-01-10T12:06:05Z
dc.date.available2024-01-10T12:06:05Z
dc.date.issued2011
dc.description.abstractBackground: Sinonasal mucosal biofilms are recognized as contributors to the pathogenesis of chronic rhinosinusitis (CRS). Attachment of bacteria to the sinonasal surface is an initial step in biofilm formation. A critical defense against this occurrence is mucociliary clearance (MCC). To ascertain whether the ciliary component of MCC is uniform throughout the airway we compared ciliary beat frequency (CBF) in the murine nasal septum and trachea at baseline and after challenge with Pseudomonas aeruginosa, a common pathogen of CRS.
dc.description.abstractMethods: Murine septal and tracheal air-liquid interface cultures were evaluated for basal and stimulated CBF after exposure to control or conditioned media from Pseudomonas. Additionally, the attachment of Pseudomonas to nasal and tracheal cultures was assessed after pretreatment with control or conditioned media.
dc.description.abstractResults: Basal CBF is significantly slower in primary nasal airway cultures compared with tracheal airway cultures. Tracheal airway cultures show resistance to Pseudomonas secreted ciliotoxins not evident in nasal septal cultures. Furthermore, after challenge with viable Pseudomonas, significantly more bacteria attach to the nasal cultures compared with the tracheal cultures.
dc.description.abstractConclusion: Using primary murine nasal and tracheal airway cultures we show inherent differences in cilia function and increased susceptibility of the upper airway to attachment by Pseudomonas. Understanding the differences between upper and subglottic airway mucociliary clearance should lead to novel approaches in the management of upper airway infection. (Am J Rhinol Allergy 25, 209-213, 2011; doi: 10.2500/ajra.2011.25.3614)
dc.fechaingreso.objetodigital2024-05-22
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.2500/ajra.2011.25.3614
dc.identifier.eissn1945-8932
dc.identifier.issn1945-8924
dc.identifier.pubmedidMEDLINE:21819755
dc.identifier.urihttps://doi.org/10.2500/ajra.2011.25.3614
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76112
dc.identifier.wosidWOS:000293136100012
dc.information.autorucMedicina;Goldstein N ;S/I;174082
dc.issue.numero4
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final213
dc.pagina.inicio209
dc.publisherSAGE PUBLICATIONS INC
dc.revistaAMERICAN JOURNAL OF RHINOLOGY & ALLERGY
dc.rightsacceso restringido
dc.subjectCHRONIC RHINOSINUSITIS
dc.subjectBEAT FREQUENCY
dc.subjectPYOCYANIN
dc.subjectSINUSITIS
dc.subjectRHINITIS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleInherent differences in nasal and tracheal ciliary function in response to Pseudomonas aeruginosa challenge
dc.typeartículo
dc.volumen25
sipa.codpersvinculados174082
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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