Multivariable optimization of ultrasound-assisted extraction for the determination of phenolic and antioxidants compounds from arrayan (Luma apiculata (DC.) Burret) leaves by microplate-based methods and mass spectrometry

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dc.article.number100356
dc.contributor.authorC.-Sandoval J.
dc.contributor.authorRodriguez A.
dc.contributor.authorH.-Aedo K.
dc.contributor.authorFalco I.
dc.contributor.authorSanchez G.
dc.contributor.authorFabra M.J.
dc.contributor.authorL.-Rubio A.
dc.contributor.authorAranda M.
dc.date.accessioned2024-01-10T12:37:07Z
dc.date.available2024-01-10T12:37:07Z
dc.date.issued2022
dc.description.abstract© 2022 Elsevier GmbHThe present study reports a multivariable optimization of ultrasound-assisted extraction of phenolic compounds from arrayan leaves. The parameters ethanol percentage in extraction solvent, temperature and extraction time, and mass to solvent ratio were optimized applying full factorial and central composite designs. According to the models, the optimal extraction conditions were: 42% of ethanol, extraction time of 27 min, extraction temperature of 50ºC and a mass to solvent ratio of 1:33.4 g mL−1. Under these conditions, a total phenolic content (TPC) of 128.16 ± 1.18 and 593.64 ± 6.49 mg of gallic acid equivalent per g of dry weight (DW) were determined in raw extract (RE) and polyphenols-rich extract (PRE), which was obtained by semi-preparative chromatography on XAD-7 column. Antioxidant capacity determined via FRAP analysis showed values of 1349.53 ± 28.99 and 6175.47 ± 127.64 µmol Trolox equivalent per g of DW for RE and PRE, and DPPH IC50 values of 831.40 ± 0.80 and 132.21 ± 2.51 µg mL−1, respectively. Polyphenols profile analyzed by liquid chromatography-mass spectrometry showed the presence of quercetin 3-ß-D-glucoside, myricetin, quercetin, kaempferol 3-glucoside and gallic acid; the latter compounds are reported for the first time in arrayan leaves. Due to the presence of polyphenols, antiviral activity was assayed over norovirus and hepatitis A showing a significant dose-dependent effect.
dc.description.funderANID
dc.description.funderNational Agency of Research and Development
dc.description.funderNational Fund for Scientific & Technological Development
dc.description.funderFONDECYT
dc.fechaingreso.objetodigital27-03-2024
dc.fuente.origenScopus
dc.identifier.doi10.1016/j.jarmap.2021.100356
dc.identifier.eissn22147861
dc.identifier.issn22147861
dc.identifier.scopusidSCOPUS_ID:85124046266
dc.identifier.urihttps://doi.org/10.1016/j.jarmap.2021.100356
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76756
dc.information.autorucFacultad de Química y de Farmacia; Aranda Bustos, Mario Antonio; S/I; 1103178
dc.language.isoen
dc.nota.accesocontenido parcial
dc.publisherElsevier GmbH
dc.relation.ispartofJournal of Applied Research on Medicinal and Aromatic Plants
dc.revistaJournal of Applied Research on Medicinal and Aromatic Plants
dc.rightsacceso restringido
dc.subjectAntiviral
dc.subjectCentral composite design
dc.subjectFull factorial design
dc.subjectLiquid chromatography
dc.subjectNutraceuticals
dc.subjectPolyphenols
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMultivariable optimization of ultrasound-assisted extraction for the determination of phenolic and antioxidants compounds from arrayan (Luma apiculata (DC.) Burret) leaves by microplate-based methods and mass spectrometry
dc.typeartículo
dc.volumen28
sipa.codpersvinculados1103178
sipa.indexScopus
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
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