Genome-wide expression profile of the response to spinal cord injury in Xenopus laevis reveals extensive differences between regenerative and non-regenerative stages

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dc.article.number12
dc.catalogadoraba
dc.contributor.authorLee Liu, D.
dc.contributor.authorMoreno, M.
dc.contributor.authorAlmonacid, L. I.
dc.contributor.authorTapia, V. S.
dc.contributor.authorMuñoz, R.
dc.contributor.authorvon Marées, J.
dc.contributor.authorGaete Carrasco, Marcia
dc.contributor.authorMelo Ledermann, Francisco Javier
dc.contributor.authorLarraín Correa, Juan Agustín
dc.date.accessioned2025-02-06T19:36:26Z
dc.date.available2025-02-06T19:36:26Z
dc.date.issued2014
dc.description.abstractBackground Xenopus laevis has regenerative and non-regenerative stages. As a tadpole, it is fully capable of functional recovery after a spinal cord injury, while its juvenile form (froglet) loses this capability during metamorphosis. We envision that comparative studies between regenerative and non-regenerative stages in Xenopus could aid in understanding why spinal cord regeneration fails in human beings. Results To identify the mechanisms that allow the tadpole to regenerate and inhibit regeneration in the froglet, we obtained a transcriptome-wide profile of the response to spinal cord injury in Xenopus regenerative and non-regenerative stages. We found extensive transcriptome changes in regenerative tadpoles at 1 day after injury, while this was only observed by 6 days after injury in non-regenerative froglets. In addition, when comparing both stages, we found that they deployed a very different repertoire of transcripts, with more than 80% of them regulated in only one stage, including previously unannotated transcripts. This was supported by gene ontology enrichment analysis and validated by RT-qPCR, which showed that transcripts involved in metabolism, response to stress, cell cycle, development, immune response and inflammation, neurogenesis, and axonal regeneration were regulated differentially between regenerative and non-regenerative stages. Conclusions We identified differences in the timing of the transcriptional response and in the inventory of regulated transcripts and biological processes activated in response to spinal cord injury when comparing regenerative and non-regenerative stages. These genes and biological processes provide an entry point to understand why regeneration fails in mammals. Furthermore, our results introduce Xenopus laevis as a genetic model organism to study spinal cord regeneration.
dc.format.extent4 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.1186/1749-8104-9-12
dc.identifier.eissn1749-8104
dc.identifier.pubmedid24885550
dc.identifier.pubmedidPMC4046850
dc.identifier.scopusid2-s2.0-84902086911
dc.identifier.urihttps://doi.org/10.1186/1749-8104-9-12
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102186
dc.identifier.wosidWOS:000336811100001
dc.information.autorucEscuela de Medicina; Gaete Carrasco, Marcia; 0000-0003-1846-2417; 1007357
dc.information.autorucFacultad de Ciencias Biológicas; Melo Ledermann, Francisco Javier; 0000-0002-0424-5991; 82342
dc.information.autorucFacultad de Ciencias Biológicas; Larraín Correa, Juan Agustín; S/I; 90468
dc.issue.numeroNo. 12
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final12
dc.pagina.inicio9
dc.revistaNeural development
dc.rightsacceso abierto
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGenome-wide expression profile of the response to spinal cord injury in Xenopus laevis reveals extensive differences between regenerative and non-regenerative stages
dc.typeartículo
dc.volumen9
sipa.codpersvinculados1007357
sipa.codpersvinculados82342
sipa.codpersvinculados90468
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