Age-dependent protein modifications and declining proteasome activity in the human lens

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dc.contributor.authorViteri, G
dc.contributor.authorCarrard, G
dc.contributor.authorBirlouez Aragon, I
dc.contributor.authorSilva, E
dc.contributor.authorFriguet, B
dc.date.accessioned2024-01-10T13:45:40Z
dc.date.available2024-01-10T13:45:40Z
dc.date.issued2004
dc.description.abstractThe proteasome is known to be the main enzymatic complex responsible for the intracellular degradation of altered proteins, and the age-related accumulation of modified lens proteins is associated to the formation of cataracts. The aim of this study was to determine whether the human lens proteasome becomes functionally impaired with age. The soluble and insoluble protein fractions of human lenses corresponding to various age-groups were characterized in terms of their levels of glyco-oxidative damage and found to show increasing anti-carboxymethyl-lysine immunoreactivity with age. Concomitantly, decreasing proteasome contents and peptidase activities were observed in the water-soluble fraction. The fact that peptidylglutamyl-peptide hydrolase activity is most severely affected with age suggests that specific changes are undergone by the proteasome itself. In particular, increasing levels of carboxymethylation were observed with age in the proteasome. It was concluded that the lower levels of soluble active enzymatic complex present in elderly lenses and the post-translational modifications affecting the proteasome may at least partly explain the decrease in proteasome activity and the concomitant accumulation of carboxymethylated and ubiquitinated proteins which occur with age. (C) 2004 Elsevier Inc. All rights reserved.
dc.fechaingreso.objetodigital2024-03-18
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.abb.2004.05.006
dc.identifier.eissn1096-0384
dc.identifier.issn0003-9861
dc.identifier.pubmedidMEDLINE:15196994
dc.identifier.urihttps://doi.org/10.1016/j.abb.2004.05.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79064
dc.identifier.wosidWOS:000222324500009
dc.information.autorucQuímica;Silva E;S/I;98771
dc.issue.numero2
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final203
dc.pagina.inicio197
dc.publisherELSEVIER SCIENCE INC
dc.revistaARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
dc.rightsacceso restringido
dc.subjectproteasome
dc.subjecthuman eye lens
dc.subjectaging
dc.subjectprotein glycation
dc.subjectCROSS-LINKED PROTEIN
dc.subjectASCORBIC-ACID
dc.subjectOXIDIZED PROTEINS
dc.subjectRAT-LIVER
dc.subjectPATHWAY
dc.subjectINHIBITION
dc.subjectGLYCATION
dc.subjectOXIDATION
dc.subject20S
dc.subjectGLUCOSE-6-PHOSPHATE-DEHYDROGENASE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAge-dependent protein modifications and declining proteasome activity in the human lens
dc.typeartículo
dc.volumen427
sipa.codpersvinculados98771
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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