The Adsorption of P2X2 Receptors Interacting with IgG Antibodies Revealed by Combined AFM Imaging and Mechanical Simulation

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dc.contributor.authorSantander, Eduardo A.
dc.contributor.authorBravo, Graciela
dc.contributor.authorChang-Halabi, Yuan
dc.contributor.authorOlguin-Orellana, Gabriel J.
dc.contributor.authorNaulin, Pamela A.
dc.contributor.authorBarrera, Mario J.
dc.contributor.authorMontenegro, Felipe A.
dc.contributor.authorBarrera, Nelson P.
dc.date.accessioned2025-01-20T17:09:55Z
dc.date.available2025-01-20T17:09:55Z
dc.date.issued2024
dc.description.abstractThe adsorption of proteins onto surfaces significantly impacts biomaterials, medical devices, and biological processes. This study aims to provide insights into the irreversible adsorption process of multiprotein complexes, particularly focusing on the interaction between anti-His6 IgG antibodies and the His6-tagged P2X2 receptor. Traditional approaches to understanding protein adsorption have centered around kinetic and thermodynamic models, often examining individual proteins and surface coverage, typically through Molecular Dynamics (MD) simulations. In this research, we introduce a computational approach employing Autodesk Maya 3D software for the investigation of multiprotein complexes' adsorption behavior. Utilizing Atomic Force Microscopy (AFM) imaging and Maya 3D-based mechanical simulations, our study yields real-time structural and kinetic observations. Our combined experimental and computational findings reveal that the P2X2 receptor-IgG antibody complex likely undergoes absorption in an 'extended' configuration. Whereas the P2X2 receptor is less adsorbed once is complexed to the IgG antibody compared to its individual state, the opposite is observed for the antibody. This insight enhances our understanding of the role of protein-protein interactions in the process of protein adsorption.
dc.description.funderFONDECYT
dc.fuente.origenWOS
dc.identifier.doi10.3390/ijms25010336
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://doi.org/10.3390/ijms25010336
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91075
dc.identifier.wosidWOS:001140644300001
dc.issue.numero1
dc.language.isoen
dc.revistaInternational journal of molecular sciences
dc.rightsacceso restringido
dc.subjectprotein adsorption
dc.subjectP2X receptor
dc.subjectprotein-protein interaction
dc.subjectmembrane protein
dc.subjectAFM imaging
dc.subjectmechanical simulation
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleThe Adsorption of P2X2 Receptors Interacting with IgG Antibodies Revealed by Combined AFM Imaging and Mechanical Simulation
dc.typeartículo
dc.volumen25
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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