Molecular characterization of a deletion/duplication rearrangement in <i>tfd</i> genes from <i>Ralstonia eutropha</i> JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid

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dc.contributor.authorClément, P
dc.contributor.authorPieper, DH
dc.contributor.authorGonzález, B
dc.date.accessioned2025-01-21T01:30:46Z
dc.date.available2025-01-21T01:30:46Z
dc.date.issued2001
dc.description.abstractRalstonia eutropha JMP134(pJP4) is able to grow on minimal media containing the pollutants 3-chlorobenzoate (3-CB) or 2,4-dichlorophenoxyacetate (2,4-D). tfd genes from the 88 kb plasmid pJP4 encode enzymes involved in the degradation of these compounds. During growth of strain JMP134 in liquid medium containing 3-CB, a derivative strain harbouring a similar to 95 kb plasmid was isolated. This derivative, designated JMP134(pJP4-F3), had an improved ability to grow on 3-CB, but had lost the ability to grow on 2,4-D. Sequence analysis of pJP4-F3 indicated that the plasmid had undergone a deletion of similar to 16 kb, which included the tfdA-tfdS intergenic region, spanning the tfdA gene to a previously unreported IS1071 element. The loss of the tfdA gene explains the failure of the derivative to grow on 2,4-D. A similar to 23 kb duplication of the region spanning tfdR-tfdD(II)C(II)E(II)F(II)-tfdB(II)-tfdK-ISJP4-tfdT-tfdC(I)D(I)E(I)F(I)-tfdB(I), giving rise to a 51-kb-long inverted repeat, was also observed. The increase in gene copy number for the tfdCD(DC)EF gene cluster may provide an explanation for the derivative strain's improved growth on 3-CB. These observations are additional examples of the metabolic plasticity of R. eutropha JMP134, one of the more versatile pollutant-degrading bacteria.
dc.fuente.origenWOS
dc.identifier.issn1350-0872
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96887
dc.identifier.wosidWOS:000170541100015
dc.language.isoen
dc.pagina.final2148
dc.pagina.inicio2141
dc.revistaMicrobiology-sgm
dc.rightsacceso restringido
dc.subjectchloroaromatics
dc.subjectcatabolic plasmid
dc.subjectIS1071 insertion sequence
dc.subjectchlorocatechol pathway
dc.titleMolecular characterization of a deletion/duplication rearrangement in <i>tfd</i> genes from <i>Ralstonia eutropha</i> JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid
dc.typeartículo
dc.volumen147
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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