Overexpression of amyloid precursor protein increases copper content in HEK293 cells

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dc.catalogadorjlo
dc.contributor.authorSuazo, Miriam
dc.contributor.authorHodar, Christián
dc.contributor.authorMorgan, Carlos
dc.contributor.authorCerpa Nebott, Francisco Waldo
dc.contributor.authorCambiazo, Verónica
dc.contributor.authorInestrosa Cantín, Nibaldo
dc.contributor.authorGonzález, Mauricio
dc.date.accessioned2024-01-29T17:48:31Z
dc.date.available2024-01-29T17:48:31Z
dc.date.issued2009
dc.description.abstractAmyloid precursor protein (APP) is a transmembrane glycoprotein widely expressed in mammalian tissues and plays a central role in Alzheimer’s disease. However, its physiological function remains elusive. Cu2+ binding and reduction activities have been described in the extracellular APP135–156 region, which might be relevant for cellular copper uptake and homeostasis. Here, we assessed Cu2+ reduction and 64Cu uptake in two human HEK293 cell lines overexpressing APP. Our results indicate that Cu2+ reduction increased and cells accumulated larger levels of copper, maintaining cell viability at supra-physiological levels of Cu2+ ions. Moreover, wild-type cells exposed to both Cu2+ ions and APP135–155 synthetic peptides increased copper reduction and uptake. Complementation of function studies in human APP751 transformed Fre1 defective Saccharomyces cerevisiae cells rescued low Cu2+ reductase activity and increased 64Cu uptake. We conclude that Cu2+ reduction activity of APP facilitates copper uptake and may represent an early step in cellular copper homeostasis.
dc.fuente.origenORCID-ene24
dc.identifier.doi10.1016/j.bbrc.2009.03.096
dc.identifier.issn1090-2104
dc.identifier.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-64749108284&partnerID=MN8TOARS
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/81032
dc.information.autorucFacultad de Ciencias Biológicas; Cerpa Nebott, Waldo Francisco; 0000-0001-7344-0144; 16584
dc.information.autorucFacultad de Ciencias Biológicas; Inestrosa Cantín, Nibaldo Manuel; 0000-0003-3118-9726; 99331
dc.issue.numero4
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final744
dc.pagina.inicio740
dc.revistaBiochemical and Biophysical Research Communications
dc.rightsacceso restringido
dc.subjectAlzheimer
dc.subjectCopper homeostasis
dc.subjectReduction
dc.subjectUptake
dc.subjectYeast
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.titleOverexpression of amyloid precursor protein increases copper content in HEK293 cells
dc.typeartículo
dc.volumen382
sipa.codpersvinculados16584
sipa.codpersvinculados99331
sipa.trazabilidadORCID;2024-01-15
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