In vitro shoot and root organogenesis, plant regeneration and production of tropane alkaloids in some species of <i>Schizanthus</i>

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dc.contributor.authorJordan, M
dc.contributor.authorHumam, M
dc.contributor.authorBieri, S
dc.contributor.authorChristen, P
dc.contributor.authorPoblete, E
dc.contributor.authorMuñoz, O
dc.date.accessioned2025-01-21T01:06:18Z
dc.date.available2025-01-21T01:06:18Z
dc.date.issued2006
dc.description.abstractA rapid in vitro propagation system leading to formation of shoots from callus, roots, and plantlets was developed for Schizanthus hookeri Gill. (Solanaceae), an endemic Chilean plant. The genus Schizanthus is of particular interest due to the presence of several tropane alkaloids. So far, in vitro propagation of species of this genus has not been reported. Propagation of S. hookeri consisted of two phases, the first one for callus initiation and shoot formation and the second for rhizogenesis and plantlet regeneration. From a single callus that rapidly increased in cell biomass (from similar to 50 mg to similar to 460 mg/culture tube [25 x 130 mm] in 60 days) in the presence of 2.69 mu M NAA and 2.22 mu M BA, more than 10 shoots/callus explant were formed. From the latter, approx. twenty plantlets formed after 90-110 days shoot subculture in medium devoid of growth regulators that favored root formation. Ten alkaloids ranging from simple pyrrolidine derivatives to tropane esters derived from angelic, tiglic, senecioic or methylmesaconic acids were obtained from in vitro regenerated plantlets. One of thern, 3 alpha-methylmesaconyloxytropane was not previously described. The same growth conditions, as well as other growth regulator levels tested, were required to induce callus and root formation in S. grahamii Gill. Root organogenesis occurred despite a high level of BA vs. NAA used, (i.e., 4.44 mu M BA and 0.54 mu M NAA); however no shoot formation was achieved. In the case of S. tricolor Grau et Gronbach, only callus formation was obtained in the presence of various growth regulators. (c) 2005 Elsevier Ltd. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.phytochem.2005.12.007
dc.identifier.issn0031-9422
dc.identifier.urihttps://doi.org/10.1016/j.phytochem.2005.12.007
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96130
dc.identifier.wosidWOS:000236163300007
dc.issue.numero6
dc.language.isoen
dc.pagina.final578
dc.pagina.inicio570
dc.revistaPhytochemistry
dc.rightsacceso restringido
dc.subjectSchizanthus hookeri
dc.subjectS. grahamii
dc.subjectS. tricolor
dc.subjectSolanaceae
dc.subjectorganogenesis
dc.subjectin vitro propagation
dc.subjecttropane alkaloid content
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIn vitro shoot and root organogenesis, plant regeneration and production of tropane alkaloids in some species of <i>Schizanthus</i>
dc.typeartículo
dc.volumen67
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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