Pregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation

Simple item page
dc.contributor.authorFarias-Jofre, Marcelo
dc.contributor.authorRomero, Roberto
dc.contributor.authorGalaz, Jose
dc.contributor.authorXu, Yi
dc.contributor.authorTao, Li
dc.contributor.authorDemery-Poulos, Catherine
dc.contributor.authorArenas-Hernandez, Marcia
dc.contributor.authorBhatti, Gaurav
dc.contributor.authorLiu, Zhenjie
dc.contributor.authorKawahara, Naoki
dc.contributor.authorKanninen, Tomi
dc.contributor.authorShaffer, Zachary
dc.contributor.authorChaiworapongsa, Tinnakorn
dc.contributor.authorTheis, Kevin R.
dc.contributor.authorTarca, Adi L.
dc.contributor.authorGomez-Lopez, Nardhy
dc.date.accessioned2024-01-10T12:37:11Z
dc.date.available2024-01-10T12:37:11Z
dc.date.issued2022
dc.description.abstractObjective To comprehensively characterize monocyte and neutrophil responses to E. coli and its product [lipopolysaccharide (LPS) or endotoxin] in vitro during pregnancy.
dc.description.abstractMaterial or subjects Peripheral blood was collected from pregnant women during the third trimester (n = 20) and from non-pregnant women (n = 20).
dc.description.abstractMethods The number, phagocytic activity, and reactive oxygen species (ROS) production of peripheral monocytes and neutrophils were investigated using flow cytometry. The phenotypes of peripheral monocytes and neutrophils after acute or chronic LPS stimulation were also determined using flow cytometry. Cytokine profiles were quantified for LPS-stimulated peripheral blood mononuclear cells (PBMCs) and a whole blood TruCulture (R) system using a multiplex immunoassay.
dc.description.abstractResults Increased number, phagocytic activity, and ROS production capacity of monocytes and neutrophils were found in pregnant compared to non-pregnant women. Additionally, specific subsets of pro-inflammatory monocytes (IL-6(+)CD14(+) or MIP-1 alpha(+)CD14(+) cells) and neutrophils (IL-1 beta(+)CD15(+) or MIP-1 beta(+)CD15(+) cells) were increased in pregnant women in response to acute LPS stimulation. Moreover, distinct subsets of intermediate-activated monocytes expressing CD142, IL-6, and IL-1RA were increased in pregnant women upon chronic LPS stimulation. Last, pregnant women displayed a different cytokine profile than non-pregnant women in LPS-stimulated PBMCs and in whole blood.
dc.description.abstractConclusions Pregnancy tailors the immune responses of circulating monocytes and neutrophils to endotoxin, a Gram-negative bacterial product.
dc.fechaingreso.objetodigital2024-04-18
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00011-022-01569-z
dc.identifier.eissn1420-908X
dc.identifier.issn1023-3830
dc.identifier.urihttps://doi.org/10.1007/s00011-022-01569-z
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76779
dc.identifier.wosidWOS:000786495600001
dc.information.autorucFacultad de Medicina; Farias Jofre, Marcelo Enrique; S/I; 12286
dc.issue.numero5-6
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final668
dc.pagina.inicio653
dc.publisherSPRINGER BASEL AG
dc.rightsacceso restringido
dc.subjectChorioamnionitis
dc.subjectCytokine
dc.subjectFunisitis
dc.subjectInfection
dc.subjectInflammation
dc.subjectPhagocytosis
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titlePregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation
dc.typeartículo
dc.volumen71
sipa.codpersvinculados12286
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Pregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation.pdf
Size:
3.2 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas