A Quinoa Protein Hydrolysate Fractionated by Electrodialysis with Ultrafiltration Membranes Improves Maternal and Fetal Outcomes in a Mouse Model of Gestational Diabetes Mellitus

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dc.contributor.authorBusso, Dolores
dc.contributor.authorGonzalez, Adrian
dc.contributor.authorSantander, Nicolas
dc.contributor.authorSaavedra, Fujiko
dc.contributor.authorQuiroz, Alonso
dc.contributor.authorRivera, Katherine
dc.contributor.authorGonzalez, Javier
dc.contributor.authorOlmos, Pablo
dc.contributor.authorMarette, Andre
dc.contributor.authorBazinet, Laurent
dc.contributor.authorIllanes, Sebastian
dc.contributor.authorEnrione, Javier
dc.date.accessioned2025-01-20T20:07:01Z
dc.date.available2025-01-20T20:07:01Z
dc.date.issued2023
dc.description.abstractScopeQuinoa intake exerts hypoglycemic and hypolipidemic effects in animals and humans. Although peptides from quinoa inhibit key enzymes involved in glucose homeostasis in vitro, their in vivo antidiabetic properties have not been investigated.Methods and resultsThis study evaluated the effect of oral administration of a quinoa protein hydrolysate (QH) produced through enzymatic hydrolysis and fractionation by electrodialysis with ultrafiltration membrane (EDUF) (FQH) on the metabolic and pregnancy outcomes of Lepdb/+ pregnant mice, a preclinical model of gestational diabetes mellitus. The 4-week pregestational consumption of 2.5 mg mL-1 of QH in water prevented glucose intolerance and improves hepatic insulin signaling in dams, also reducing fetal weights. Sequencing and bioinformatic analyses of the defatted FQH (FQHD) identified 11 peptides 6-10 amino acids long that aligned with the quinoa proteome and exhibited putative anti-dipeptidyl peptidase-4 (DPP-IV) activity, confirmed in vitro in QH, FQH, and FDQH fractions. Peptides homologous to mouse and human proteins enriched for biological processes related to glucose metabolism are also identified.ConclusionProcessing of quinoa protein may be used to develop a safe and effective nutritional intervention to control glucose intolerance during pregnancy. Further studies are required to confirm if this nutritional intervention is applicable to pregnant women.
dc.description.abstractThis study evaluated the effect of oral administration of a quinoa protein hydrolysate produced through enzymatic hydrolysis and fractionation (FQH) on metabolic and pregnancy outcomes of Lepdb/+ pregnant mice, a preclinical model of gestational diabetes mellitus (GDM). Consumption of FQH prevented glucose intolerance, reduce hepatic triglyceride (TG) levels, and promoted liver insulin signaling in mothers and reduced the fetal weight. image
dc.fuente.origenWOS
dc.identifier.doi10.1002/mnfr.202300047
dc.identifier.eissn1613-4133
dc.identifier.issn1613-4125
dc.identifier.urihttps://doi.org/10.1002/mnfr.202300047
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91753
dc.identifier.wosidWOS:001058185900001
dc.issue.numero21
dc.language.isoen
dc.revistaMolecular nutrition & food research
dc.rightsacceso restringido
dc.subjectelectrodialysis with filtration membrane
dc.subjectgestational diabetes mellitus
dc.subjectmice
dc.subjectpeptides
dc.subjectprotein hydrolysate
dc.subjectquinoa
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleA Quinoa Protein Hydrolysate Fractionated by Electrodialysis with Ultrafiltration Membranes Improves Maternal and Fetal Outcomes in a Mouse Model of Gestational Diabetes Mellitus
dc.typeartículo
dc.volumen67
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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