CryoEM analysis of small plant biocatalysts at sub-2 Å resolution

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dc.contributor.authorDimos, Nicole
dc.contributor.authorHelmer, Carl P. O.
dc.contributor.authorChanique, Andrea M.
dc.contributor.authorWahl, Markus C.
dc.contributor.authorKourist, Robert
dc.contributor.authorHilal, Tarek
dc.contributor.authorLoll, Bernhard
dc.date.accessioned2025-01-20T22:01:17Z
dc.date.available2025-01-20T22:01:17Z
dc.date.issued2022
dc.description.abstractEnzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of similar to 120 kDa at or below 2 angstrom resolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.
dc.fuente.origenWOS
dc.identifier.doi10.1107/S205979832101216X
dc.identifier.issn2059-7983
dc.identifier.urihttps://doi.org/10.1107/S205979832101216X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93805
dc.identifier.wosidWOS:000738234900011
dc.language.isoen
dc.pagina.final123
dc.pagina.inicio113
dc.revistaActa crystallographica section d-structural biology
dc.rightsacceso restringido
dc.subjectcryo-electron microscopy
dc.subjectcamphor
dc.subjectterpenes
dc.subjectborneol dehydrogenases
dc.subjecthigh resolution
dc.subjectgreen chemistry
dc.subjectplant biocatalysts
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCryoEM analysis of small plant biocatalysts at sub-2 Å resolution
dc.typeartículo
dc.volumen78
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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