Genome, transcriptome, and secretome analysis of wood decay fungus <i>Postia placenta</i> supports unique mechanisms of lignocellulose conversion

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dc.contributor.authorMartinez, Diego
dc.contributor.authorChallacombe, Jean
dc.contributor.authorMorgenstern, Ingo
dc.contributor.authorHibbett, David
dc.contributor.authorSchmoll, Monika
dc.contributor.authorKubicek, Christian P.
dc.contributor.authorFerreira, Patricia
dc.contributor.authorRuiz-Duenas, Francisco J.
dc.contributor.authorMartinez, Angel T.
dc.contributor.authorKersten, Phil
dc.contributor.authorHammel, Kenneth E.
dc.contributor.authorWymelenberg, Amber Vanden
dc.contributor.authorGaskell, Jill
dc.contributor.authorLindquist, Erika
dc.contributor.authorSabat, Grzegorz
dc.contributor.authorBonDurant, Sandra Splinter
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCanessa, Paulo
dc.contributor.authorVicuna, Rafael
dc.contributor.authorYadav, Jagjit
dc.contributor.authorDoddapaneni, Harshavardhan
dc.contributor.authorSubramanian, Venkataramanan
dc.contributor.authorPisabarro, Antonio G.
dc.contributor.authorLavin, Jose L.
dc.contributor.authorOguiza, Jose A.
dc.contributor.authorMaster, Emma
dc.contributor.authorHenrissat, Bernard
dc.contributor.authorCoutinho, Pedro M.
dc.contributor.authorHarris, Paul
dc.contributor.authorMagnuson, Jon Karl
dc.contributor.authorBaker, Scott E.
dc.contributor.authorBruno, Kenneth
dc.contributor.authorKenealy, William
dc.contributor.authorHoegger, Patrik J.
dc.contributor.authorKuees, Ursula
dc.contributor.authorRamaiya, Preethi
dc.contributor.authorLucash, Susan
dc.contributor.authorSalamov, Asaf
dc.contributor.authorShapiro, Harris
dc.contributor.authorTu, Hank
dc.contributor.authorChee, Christine L.
dc.contributor.authorMisra, Monica
dc.contributor.authorXie, Gary
dc.contributor.authorTeter, Sarah
dc.contributor.authorYaver, Debbie
dc.contributor.authorJames, Tim
dc.contributor.authorMokrejs, Martin
dc.contributor.authorPospisek, Martin
dc.contributor.authorGrigoriev, Igor V.
dc.contributor.authorBrettin, Thomas
dc.contributor.authorRokhsar, Dan
dc.contributor.authorBerka, Randy
dc.contributor.authorCullen, Dan
dc.date.accessioned2025-01-21T00:09:38Z
dc.date.available2025-01-21T00:09:38Z
dc.date.issued2009
dc.description.abstractBrown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exo-cellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative beta-1-4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also up-regulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H2O2. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe( II) and H2O2 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.
dc.fuente.origenWOS
dc.identifier.doi10.1073/pnas.0809575106
dc.identifier.issn0027-8424
dc.identifier.urihttps://doi.org/10.1073/pnas.0809575106
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95716
dc.identifier.wosidWOS:000263252500052
dc.issue.numero6
dc.language.isoen
dc.pagina.final1959
dc.pagina.inicio1954
dc.revistaProceedings of the national academy of sciences of the united states of america
dc.rightsacceso restringido
dc.subjectcellulose
dc.subjectfenton
dc.subjectlignin
dc.subjectcellulase
dc.subjectbrown-rot
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleGenome, transcriptome, and secretome analysis of wood decay fungus <i>Postia placenta</i> supports unique mechanisms of lignocellulose conversion
dc.typeartículo
dc.volumen106
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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