Stress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic <i>Bacillus</i> Species from Chilean Hot Spring

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dc.contributor.authorMarin-Sanhueza, Cathalina
dc.contributor.authorEcheverria-Vega, Alex
dc.contributor.authorGomez, Aleydis
dc.contributor.authorCabrera-Barjas, Gustavo
dc.contributor.authorRomero, Romina
dc.contributor.authorBanerjee, Aparna
dc.date.accessioned2025-01-20T21:12:10Z
dc.date.available2025-01-20T21:12:10Z
dc.date.issued2022
dc.description.abstractThermophilic bacteria able to survive extreme temperature stress are of great biotechnological interest due to their extracellular production of bioactive molecules as a part of a survival strategy, or by intracellular modifications. In the present study, thermophilic Bacillus haynesii CamB6, isolated from a Chilean hot spring, was studied for the formation of different stress response molecules. The polymeric pigment produced by the bacterial strain was characterized by different physicochemical techniques. On exposure to ranges of temperature (50-60 degrees C), pH (5.0-7.0), and sources of nitrogen and carbon (1-5 g center dot L-1), the bacteria responded with a biofilm network formation in a hydrophobic polystyrene surface. Biofilm formation under fed-batch conditions was also statistically validated. The bacteria showed a planktonic pellicle network formation in the presence of induced hypoxia and salinity stress (19.45 g center dot L-1) under static conditions. Salinity stress also resulted in the intracellular response of brown pigment production. The pigment was structurally and functionally characterized by UV-Vis absorbance and the presence of different characteristic peaks via FTIR analysis (bacterial pyomelanin fingerprints) were assessed. A high thermal stability and TGA profile indicated the brown pigment was a probable pyomelanin candidate. Micropyrolysis (Py-GC/MS) showed that isoprene, pyrrole, benzene, pyridine, and their derivatives were the major components detected. In addition, acetic acid, indole, phenol, and its derivatives were observed. The absence of sulfocompounds in the pyrolyzed products agreed with those reported in the literature for pyomelanin. The pigment surface morphology was analyzed via SEM, and the elemental composition via EDS also demonstrated the similarity of the brown pigment to that of the melanin family. The pyomelanin pigment was observed to be bioactive with promising antioxidant capacity (H2O2, Fe2+) compared to the standard antioxidant molecules. In conclusion, B. haynesii CamB6 demonstrated the formation of several biomolecules as a stress response mechanism that is bioactive, showing its probable biotechnological applications in future.
dc.fuente.origenWOS
dc.identifier.doi10.3390/polym14040680
dc.identifier.eissn2073-4360
dc.identifier.urihttps://doi.org/10.3390/polym14040680
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93647
dc.identifier.wosidWOS:000765129100001
dc.issue.numero4
dc.language.isoen
dc.revistaPolymers
dc.rightsacceso restringido
dc.subjectextremophile
dc.subjectBacillus
dc.subjectbiofilm
dc.subjectpellicle
dc.subjectpigment
dc.subjectmelanin
dc.subjectantioxidant
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleStress Dependent Biofilm Formation and Bioactive Melanin Pigment Production by a Thermophilic <i>Bacillus</i> Species from Chilean Hot Spring
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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