Increased placental angiogenesis in late and early onset pre-eclampsia is associated with differential activation of vascular endothelial growth factor receptor 2

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dc.contributor.authorEscudero, C.
dc.contributor.authorCelis, C.
dc.contributor.authorSaez, T.
dc.contributor.authorSan Martin, S.
dc.contributor.authorValenzuela, F. J.
dc.contributor.authorAguayo, C.
dc.contributor.authorBertoglia, P.
dc.contributor.authorRoberts, J. M.
dc.contributor.authorAcurio, J.
dc.date.accessioned2024-01-10T12:39:42Z
dc.date.available2024-01-10T12:39:42Z
dc.date.issued2014
dc.description.abstractIntroduction: Placentas from both early-onset (EOPE) and late-onset pre-eclampsia (LOPE) exhibit signs of underperfusion, which in turn, may be associated with altered angiogenesis. Tyrosine 951 (Y951) and Y1175 phosphorylation of the vascular endothelial growth factor receptor 2 (VEGFR2) induced by VEGF triggers the angiogenesis process. Endothelial markers such as CD31 and CD34 have been used for estimating angiogenic processes in several tissues, including placenta. We asked whether vascular density in placental villi was related to Y951/Y1175 phosphorylation of VEGFR2 in LOPE or EOPE.
dc.description.abstractMethods: We obtained placental samples from women with normal pregnancies (n = 22), LOPE (n = 13), EOPE (n = 15) and preterm deliveries (n = 10). Slices from placental tissue were used for CD31 immunostaining. We estimated the expression of CD31, CD34, VEGF, and VEGFR2 by western blot and quantitative PCR. Y951 phosphorylation of VEGFR2 was estimated by western blot, whereas Y1175 phosphorylation was analyzed by ELISA.
dc.description.abstractResults: Vessel density in terminal villi and CD31 and CD34 protein abundance were increased in LOPE and EOPE compared to normal pregnancy. However, mRNA levels for CD31 and CD34 were lower in LOPE than in normal pregnancy and VEGF mRNA was higher in EOPE. VEGFR2 protein concentration was not different among the studied groups. Y951 and Y1175 phosphorylation of VEGFR2 was higher in LOPE than in the normotensive group, but only Y951 exhibited greater phosphorylation in EOPE compared to normal pregnancy. Discussion: Changes in vessel formation in the pre-eclamptic placenta are controversial. Our study suggests a pro-angiogenic state in both LOPE and EOPE. These changes are however, associated with differential expression of endothelial markers and VEGFR2 activation.
dc.description.abstractConclusion: There is evidence of increased placental angiogenesis in LOPE and EOPE that is associated with differential activation of VEGFR2. (C) 2014 Elsevier Ltd. All rights reserved.
dc.description.funderMagee-Womens Research Institute Fondecyt
dc.description.funderConicyt
dc.description.funderDIUBB
dc.description.funderConicyt Anillo
dc.fechaingreso.objetodigital05-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.placenta.2014.01.007
dc.identifier.eissn1532-3102
dc.identifier.issn0143-4004
dc.identifier.pubmedidMEDLINE:24508097
dc.identifier.urihttps://doi.org/10.1016/j.placenta.2014.01.007
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77224
dc.identifier.wosidWOS:000333495900009
dc.information.autorucMedicina;Saez T;S/I;1006611
dc.issue.numero3
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final215
dc.pagina.inicio207
dc.publisherW B SAUNDERS CO LTD
dc.revistaPLACENTA
dc.rightsacceso restringido
dc.subjectAngiogenesis
dc.subjectPlacenta
dc.subjectPre-eclampsia
dc.subjectVEGFR2
dc.subjectPhosphorylation
dc.subjectFETAL-GROWTH
dc.subjectCOMPLICATED PREGNANCIES
dc.subjectMATERNAL UNDERPERFUSION
dc.subjectHYPERTENSIVE DISORDERS
dc.subjectADHESION MOLECULES
dc.subjectVEGF RECEPTOR-2
dc.subjectGENE-EXPRESSION
dc.subjectTERMINAL VILLI
dc.subjectMESSENGER-RNA
dc.subjectRESTRICTION
dc.subject.ods03 Good Health and Well-being
dc.subject.ods05 Gender Equality
dc.subject.odspa03 Salud y bienestar
dc.subject.odspa05 Igualdad de género
dc.titleIncreased placental angiogenesis in late and early onset pre-eclampsia is associated with differential activation of vascular endothelial growth factor receptor 2
dc.typeartículo
dc.volumen35
sipa.codpersvinculados1006611
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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