Comparative analysis of TMV-Cg and TMV-U1 detection methods in infected <i>Arabidopsis thaliana</i>

Simple item page
dc.contributor.authorPereda, S
dc.contributor.authorEhrenfeld, N
dc.contributor.authorMedina, C
dc.contributor.authorDelgado, J
dc.contributor.authorArce-Johnson, P
dc.date.accessioned2025-01-21T01:31:07Z
dc.date.available2025-01-21T01:31:07Z
dc.date.issued2000
dc.description.abstractThe common strain of the tobacco mosaic virus (TMV-U1), and the crucifer-infecting tobacco mosaic virus (TMV-Cg), both members of Tobamovirus genus, infect efficiently the solanaceous plants such as tomato and tobacco. The crucifer-infecting tobacco mosaic virus (TMV-Cg) also infects Arabidopsis thaliana plant, spreading systemically without causing severe symptoms. In contrast, Arabidopsis is a poor host for TMV-U1 infection. Within the past 10 years, Arabidopsis has developed into a powerful model system for studying plant-pathogen interaction. However, a detailed analysis comparing the accuracy of various viral detection methods has not been reported previously. Four detection methods were evaluated in A. thaliana (ecotype Po-1), infected with TMV-U1 or TMV-Cg. Western blots, enzyme-linked immunosorbent assay (ELISA), reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ RNA hybridization methods were used to determine Viral spread at Various days post inoculation (dpi) in inoculated and apical non-inoculated leaves. The detection of viral spread of TMV-U1 and TMV-Cg in Arabidopsis, using these four detection methods, supports previous studies, which demonstrate that the systemic spreads of these two viruses differ in Arabidopsis. Western blotting and ELISA detected TMV-Cg at 5dpi, and TMV-U1 at 12 dpi in systemic tissues. Viral spread was detected earlier when using RNA detection methods. Reverse transcriptase-polymerase chain reaction (RT-PCR) was very sensitive for detecting TMV-CS in A. thaliana, but less sensitive for TMV-U1 detection. In situ RNA hybridization showed differential distribution of TMV-Cg and TMV-U1 in the inoculated leaf and systemic tissues. (C) 2000 Elsevier Science B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.issn0166-0934
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96986
dc.identifier.wosidWOS:000165349400004
dc.issue.numero2
dc.language.isoen
dc.pagina.final142
dc.pagina.inicio135
dc.revistaJournal of virological methods
dc.rightsacceso restringido
dc.subjectTobamovirus detection
dc.subjectin situ RNA hybridization
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleComparative analysis of TMV-Cg and TMV-U1 detection methods in infected <i>Arabidopsis thaliana</i>
dc.typeartículo
dc.volumen90
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas