Dissecting the structural and functional consequences of the evolutionary proline-glycine deletion in the wing 1 region of the forkhead domain of human FoxP1

Simple item page
dc.contributor.authorTamarin, Stephanie
dc.contributor.authorGalaz-Davison, Pablo
dc.contributor.authorRamirez-Sarmiento, Cesar A.
dc.contributor.authorBabul, Jorge
dc.contributor.authorMedina, Exequiel
dc.date.accessioned2025-01-20T16:14:21Z
dc.date.available2025-01-20T16:14:21Z
dc.date.issued2024
dc.description.abstractThe human FoxP transcription factors dimerize via three-dimensional domain swapping, a unique feature among the human Fox family, as result of evolutionary sequence adaptations in the forkhead domain. This is the case for the conserved glycine and proline residues in the wing 1 region, which are absent in FoxP proteins but present in most of the Fox family. In this work, we engineered both glycine (G) and proline-glycine (PG) insertion mutants to evaluate the deletion events in FoxP proteins in their dimerization, stability, flexibility, and DNA-binding ability. We show that the PG insertion only increases protein stability, whereas the single glycine insertion decreases the association rate and protein stability and promotes affinity to the DNA ligand.
dc.fuente.origenWOS
dc.identifier.doi10.1002/1873-3468.14972
dc.identifier.eissn1873-3468
dc.identifier.issn0014-5793
dc.identifier.urihttps://doi.org/10.1002/1873-3468.14972
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/90436
dc.identifier.wosidWOS:001260154200001
dc.issue.numero18
dc.language.isoen
dc.pagina.final2291
dc.pagina.inicio2281
dc.revistaFebs letters
dc.rightsacceso restringido
dc.subjectDNA-binding affinity
dc.subjectdomain swapping
dc.subjectevolutionary mutations
dc.subjectforkhead DNA-binding proteins
dc.subjectP subfamily
dc.subjectprotein-protein interactions
dc.subjectstability
dc.subjecttranscription factor
dc.subject.ods15 Life on Land
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.titleDissecting the structural and functional consequences of the evolutionary proline-glycine deletion in the wing 1 region of the forkhead domain of human FoxP1
dc.typeartículo
dc.volumen598
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas