Unraveling the folding and dimerization properties of the human FoxP subfamily of transcription factors

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dc.contributor.authorVillalobos, Pablo
dc.contributor.authorCarvajal, Alonso I. I.
dc.contributor.authorCastro-Fernandez, Victor
dc.contributor.authorBabul, Jorge
dc.contributor.authorRamirez-Sarmiento, Cesar A.
dc.contributor.authorMedina, Exequiel
dc.date.accessioned2025-01-20T20:13:52Z
dc.date.available2025-01-20T20:13:52Z
dc.date.issued2023
dc.description.abstractHuman FoxP proteins share a highly conserved DNA-binding domain that dimerizes via three-dimensional domain swapping, although showing varying oligomerization propensities among its members. Here, we present an experimental and computational characterization of all human FoxP proteins to unravel how their amino acid substitutions impact their folding and dimerization mechanism. We solved the crystal structure of the forkhead domain of FoxP4 to then perform a comparison across all members, finding that their sequence changes impact not only the structural heterogeneity of their forkhead domains but also the protein-protein association energy barrier. Lastly, we demonstrate that the accumulation of a monomeric intermediate is an oligomerization-dependent feature rather than a common aspect of monomers and dimers in this protein subfamily.
dc.fuente.origenWOS
dc.identifier.doi10.1002/1873-3468.14665
dc.identifier.eissn1873-3468
dc.identifier.issn0014-5793
dc.identifier.urihttps://doi.org/10.1002/1873-3468.14665
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92163
dc.identifier.wosidWOS:000994674700001
dc.issue.numero14
dc.language.isoen
dc.pagina.final1905
dc.pagina.inicio1894
dc.revistaFebs letters
dc.rightsacceso restringido
dc.subjectDNA-binding proteins
dc.subjectdomain swapping
dc.subjectfolding mechanism
dc.subjectforkhead domain
dc.subjectFoxP subfamily
dc.subjectprotein-protein interactions
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleUnraveling the folding and dimerization properties of the human FoxP subfamily of transcription factors
dc.typeartículo
dc.volumen597
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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