Visible light-induced photooxidation of glucose sensitized by riboflavin
Loading...
Date
1999
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
ELSEVIER SCIENCE INC
Abstract
Ne conducted this study to evaluate the oxidation of glucose induced by visible light in the presence of sensitizers such as methylene blue and flavins (i.e., flavin mononucleotide and riboflavin). The concentration of the sensitizers was similar to that of flavin in parenteral nutrients. The photooxidation of glucose sensitized by flavin mononucleotide or riboflavin was greater than that which was observed in the presence of methylene blue, whereas the isotopic effect of deuterium oxide (D2O) was enhanced more substantially in the presence of methylene blue than in the presence of flavins. These results show that methylene blue exerts its action through singlet oxygen and that at a high substrate concentration las was used in this work flavin mononucleotide and riboflavin act preferentially, as type I sensitizers. In the flavin photosensitized processes, the presence of hydrogen peroxide, superoxide anion, and hydroxyl radical was demonstrated. The photooxidation of glucose is favored by an increase in PH, and it also depends on the energy absorbed by the system. By using a specific reagent for glucose (i.e., o-toluidine), it was possible to quantify the photoconversion of glucose. The results obtained in this work should be considered in the management of glucose-containing parenteral nutrients that are exposed to risible light in the presence of a multivitamin complex containing flavin mononucleotide. (C) Elsevier Science Inc. 1999. All rights reserved.
Description
Keywords
riboflavin, glucose, photosensitized, photooxidation, SINGLET MOLECULAR-OXYGEN, LIFETIME