Deletions of <i>mob</i> and <i>tra</i> pJP4 transfer functions after mating of <i>Ralstonia</i> <i>eutropha</i> JMP134 (pJP4) with <i>Escherichia</i> <i>coli</i> harboring F′
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2000
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Abstract
One-tenth of Escherichia coli transconjugants resulting from the transfer of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 to E. coli XL1Blue, contained pJP4 derivatives with deletions (similar to 15-30 kb). The occurrence of these deletions is probably associated with the presence of Tn10 in the recipient. DNA endonuclease restriction analysis of the pJP4 deletion derivatives showed the absence of SphI and EcoRI fragments previously reported to hybridize with IncP Tra DNA probes. Moreover, these pJP4 deletion derivatives are not able to self-transfer, nor are they able to be mobilized. Accordingly, these pJP4 deletion derivatives lack transfer functions.
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Keywords
pJP4, mobilization functions, deletion