Deletions of <i>mob</i> and <i>tra</i> pJP4 transfer functions after mating of <i>Ralstonia</i> <i>eutropha</i> JMP134 (pJP4) with <i>Escherichia</i> <i>coli</i> harboring F′

Simple item page
dc.contributor.authorClément, P
dc.contributor.authorSpringael, D
dc.contributor.authorGonzález, B
dc.date.accessioned2025-01-21T01:31:25Z
dc.date.available2025-01-21T01:31:25Z
dc.date.issued2000
dc.description.abstractOne-tenth of Escherichia coli transconjugants resulting from the transfer of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 to E. coli XL1Blue, contained pJP4 derivatives with deletions (similar to 15-30 kb). The occurrence of these deletions is probably associated with the presence of Tn10 in the recipient. DNA endonuclease restriction analysis of the pJP4 deletion derivatives showed the absence of SphI and EcoRI fragments previously reported to hybridize with IncP Tra DNA probes. Moreover, these pJP4 deletion derivatives are not able to self-transfer, nor are they able to be mobilized. Accordingly, these pJP4 deletion derivatives lack transfer functions.
dc.fuente.origenWOS
dc.identifier.eissn1480-3275
dc.identifier.issn0008-4166
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97059
dc.identifier.wosidWOS:000087257600011
dc.issue.numero5
dc.language.isoen
dc.pagina.final489
dc.pagina.inicio485
dc.revistaCanadian journal of microbiology
dc.rightsacceso restringido
dc.subjectpJP4
dc.subjectmobilization functions
dc.subjectdeletion
dc.titleDeletions of <i>mob</i> and <i>tra</i> pJP4 transfer functions after mating of <i>Ralstonia</i> <i>eutropha</i> JMP134 (pJP4) with <i>Escherichia</i> <i>coli</i> harboring F′
dc.typeartículo
dc.volumen46
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas