BIOSENSORS' PREPARATION TO BE USED FOR ENTRAPMENT METHOD WITH COPOLYMERS POLYANILINE DERIVATIVES
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Date
2010
Journal Title
Journal ISSN
Volume Title
Publisher
SOC CHILENA QUIMICA
Abstract
The entrapment method was used to prepare electrochemical biosensors. Using controlled potential method, copolymers were synthesized between aniline y sulfonated-anilines in aqueous media at pH 4.9 on Platinum disc. The copolymers were characterized by elemental analysis and spectroscopy. Glucose-oxidase was entrapped in the electropolymerization process, at 4.9 in aqueous media. The copolymers modified electrodes and copolymer/enzyme were studied as working electrodes for the oxidation of different H2O2 concentrations and indirect D-glucose quantification at pH 7, using controlled potential and the cyclic voltammetry methods (CV) correspondingly. A linear relationship between the hydrogen peroxide (or D-glucose) concentration and the electrochemical response was obtained at pH 7. The modified-electrodes displayed a fast time of current response, limited by the cycle numbers of the cyclic voltammetry, 232 s, and enough stability to allow for the measurements to be taken after several days.
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Keywords
Copolymers, Electrochemistry, enzyme, biosensor, CONDUCTING POLYMERS, GLUCOSE BIOSENSOR, OXIDASE, POLYMERIZATION, IMMOBILIZATION, FILM